CD163 Molecule (CD163) 抗体

CD163 Molecule (CD163) 抗体抗原
CD163 Molecule (CD163)
别名 DMBT1, CD163, CD163v2, CD163v3, M130, MM130
适用
其他选择 人, 猴
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (RM3-1)
标记
其他选择 非结合性
CD163 Molecule (CD163) 抗体应用范围
其他选择 Immunohistochemistry (Frozen Sections) (IHC (fro)), 流式细胞分析技术（FACS）, Western Blotting (WB)
Pubmed 2个引用可参考
规格 0.2 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb111878
产品细节
免疫原 Human monocytes, particularly after dexamethasone treatment or after 2-5 days in culture
克隆位点 RM3-1
亚型 IgG1
特异性 This antibody is specific for CD163. Clone RM 3/1 recognizes a membrane glycoprotein on monocytes and macrophages which is expressed in intermediate and late inflammatory stages. Does not react with lymphocytes, granulocytes or platelets. Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, and cortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with clone 25F9 which is not the case in other tissues.
交叉反应 （详细） Species reactivity (tested):Human, Monkey. Negative in Pig, Rabbit and Guinea Pig.
CD163 Molecule (CD163) 抗体纯化方法 Affinity Chromatography
目标详细情况
别名 CD163
背景 CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130
基因ID 9332
NCBI登录号 NP_004235
UniProt Q86VB7
研究领域 Cardiovascular, Atherosclerosis, CD Antigens, Innate Immunity, Surface Receptors of Immune Cells
使用细节
应用备注 Immunohistochemistry on Frozen Sections: 4 μg/mL (1/100). Does not work in routinely processed paraffin sections. Has been described to work in FACS and Western blots. Recommended Positive control: Human placenta.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理
溶解方式 Restore with 0.5 mL distilled water to a concentration of 0.4 mg/mL.
浓度 0.4 mg/mL
缓冲液 Stock solutions contains PBS, pH 7.2, 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
储存液 Thimerosal (Merthiolate)
注意事项 This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
CD163 Molecule (CD163) 抗体注意事项 This product is photosensitive and should be protected from light
储存条件 4 °C
储存方法 Store the antibody undiluted Prior to and after reconstitution at 2-8 °C. DO NOT FREEZE!