- ¥2500
- LONZA
- PT-3001
- 2025年08月13日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
5
- 供应商：
研卉生物
- 英文名：
MSCGM BulletKit（PT-3238&PT-4105）
- 规格：
盒
MSCGM BulletKit（PT-3238&PT-4105）
Product Overview:
Stem cells possess the unique ability to proliferate as an undifferentiated cell type, then, when properly stimulated, differentiate into multiple lineages of cells. Lonza’s MSCGM human Mesenchymal Stem Cell Growth BulletKit™ Medium is a serum containing medium designed to proliferate human bone marrow derived mesenchymal cells in an undifferentiated state. The serum used in Lonza’s MSCGM human Mesenchymal Stem Cell Growth Medium BulletKit™ Medium is specifically screened to ensure that the serum neither promotes spontaneous differentiation of cells nor inhibits cell differentiation to osteogenic, chondrogenic, or adipogenic lineages (when properly stimulated with an appropriate differentiation media).
Partial List of Cells Proliferated with MSCGM human Mesenchymal Stem Cell Growth BulletKit™ Medium*:
Human bone marrow derived stem cells
Human periodontal ligament derived stem cells
The MSCGM human Mesenchymal Stem Cell Growth BulletKit™ Medium is offered as a BulletKit™ Medium (catalog no. PT-3001) which includes both the basal media and the necessary supplements for proliferation of human bone marrow derived mesenchymal stem cells. The basal medium (MSCBM hMSC Basal Medium; catalog no. PT-3238) and the necessary supplements (MSCGM hMSC SingleQuot Kit; catalog no. PT-4105) can also be purchased individually.
Trypsin/EDTA (catalog no. CC-3232): Lonza's recommended Trypsin/EDTA for all strongly adherent primary cells including normal human articular chondrocytes (NHAC-Kn), human bone marrow derived mesenchymal stem cells (hMSC), rat bone marrow derived mesenchymal stem cells (rMSC), and dental pulp stem cells (DPSC).
Lonza guarantees the performance of Clonetics™/Poietics™ cells only if appropriate Clonetics™/Poietics™ media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.
*List of cells generated from customer data. Lonza only routinely uses this medium with Lonza’s human bone marrow derived mesenchymal stem cells and can therefore only guarantee the successful use of this media with these cells.
LONZA
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培养基类型
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产品编号
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产品名称
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规格
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间充质干细胞生长培养基
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PT-3001
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MSCGM BulletKit（PT-3238&PT-4105）
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1Kit
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人间充质干细胞软骨分化培养基
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PT-3002
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hMSC Osteogenic BulletKit（PT-3923/4120）
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1Kit
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人间充质干细胞成骨分化培养基
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PT-3004
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Hmsc Adipogenic PT3102B,3102A,4122,4135
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1Kit
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文献和实验PromoCell MSC分化培养操作步骤 一、向神经细胞分化的操作流程 1、将塑料培养器用纤维连接蛋白包被根据操作手册，用纤维连接蛋白（C-43050）包被合适的组织培养瓶。使用浓度为10μg/ml。2、接种间充质干细胞用MSC生长培养基（C-28010）在用纤维连接蛋白包被过的培养瓶中接种培养，接种密度为4000个/cm2。3、培养间充质干细胞将细胞培养至80%的饱和度，每48小时更换一次培养基。4、诱导间充质干细胞用MSCU经细胞分化培养基（C-28015）诱导培养，用MSC
-free Medium无血清培养基(Serum Free Medium, SFM)是由世界著名的美国LONZA公司生产。该12-725F UltraCULTURE Serum-free Medium无血清培养基是一种营养成分完全的通用型的无血清培养基，适用于培养贴壁哺乳动物细胞和悬浮哺乳动物细胞;适于在疫苗生产中制备病毒颗粒;支持杂交瘤形成时的细胞融合，支持单核细胞、巨噬细胞、上皮细胞和成纤维细胞系的细胞生长。适合多种细胞类型的无血清培养，包括HeLa细胞、HEC细胞、HEL,N-10细胞、HNK细胞
分离培养基上菌落的生长现象是检验主管技师考试辅导的部分内容，以下是医学教育网对这块内容的整理，希望对考生有所帮助： 1.观察菌落：菌落的各种特征包括大小、形状、突起、边缘、颜色、表面、透明度和粘度等。 根据细菌菌落表面特征不同，可将菌落分为三型： （1）光滑型（S型）菌落 （2）粗糙型（R型）菌落 （3）粘液型（M型）菌落 2.血琼脂上的溶血 α溶血：又叫草绿色溶血，菌落周围血培养基变为绿色
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