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- 英文名:
hMSC Adipogenic Differentiation BulletKit™ Medium
- 规格:
kit
hMSC Adipogenic Differentiation BulletKit™ Medium
属性:hMSC分化培养基
Shelf Life(month):12
产地:USA
存储条件:2-8
A serum containing media system designed to induce adipogenic differentiation of human bone marrow derived mesenchymal stem cells into mature, functionally active adipocytes in as few as 19 days. Differentiation can then be validated by a variety of methods including the use of Lonza’s AdipoRed™ Adipogenic Assay Reagent which provides a quantitative value related to the amount of adipogenic differentiation.
The hMSC Adipogenic Differentiation BulletKit™ Medium provides both an induction medium and a maintenance medium guaranteed to induce adipogenic differentiation of human bone marrow derived mesenchymal stem cells into mature, functionally active adipocytes; Each bottle of the hMSC Adipogenic Differentiation BulletKit™ Medium contains enough induction medium and maintenance medium to fully differentiate approximately 3 million human bone marrow derived mesenchymal stem cells [approximately 15 wells in a 6-well plate format] into mature, functionally active adipocytes.
The hMSC Adipogenic Differentiation Medium is offered as a BulletKit™ Medium (catalog no. PT-3004) which includes both the basal media for induction and maintenance and the necessary supplements for both adipogenic induction and maintenance of human bone marrow derived mesenchymal stem cells.
Partial List of Cells Differentiated with hMSC Adipogenic Differentiation BulletKit™ Medium*:
Human bone marrow derived stem cells
C57BL/6 mice bone marrow derived stem cells
human adipose derived stem cells
Human periodontal ligament derived stem cells
包装:维持培养基170ml;诱导培养基170ml;维持添加剂包括胰岛素、L-谷氨酰胺、MCGS、GA-1000;诱导添加剂包括胰岛素、L-谷氨酰胺、MCGS、地塞米松、吲哚美辛、IBMX、GA-1000
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文献和实验R&D Systems:如何提高MSC的扩增且不损失其分化潜能
培养于盖玻片上的细胞20分钟,然后室温下用含有10%标准驴血清,0.3%Triton X-100和1%BSA的PBS封闭45分钟。封闭后,在2至8℃下用稀释的一抗孵育细胞过夜,然后在室温下的暗室中用NorthernLights-557连接的二抗孵育细胞一个小时。每一个步骤间,细胞用含有0.1%BSA的PBS清洗三次。 成脂分化。按人骨髓间充质干细胞功能鉴定试剂盒的说明书诱导hMSC分化为脂肪细胞,骨细胞或软骨细胞。简而言之,准备完全成脂分化培养基和带有无菌盖玻片的24孔组织
内芽体就会自发形成。 3、诱导间充质干细胞芽体 将其中一份培养的细胞用MSC成软骨分化培养基诱导,另一份培养的细胞用MSC生长培养基培养作为阴性对照。4、诱导得到的间充质干细胞芽体的分化培养 诱导培养14天,每3天更换一次培养基。操作时候小心不要吸到芽体。要点:在整个染色过程中不要让细胞在干的状态下放置超过30秒! 四、向成脂分化和操作流程 1、接种间充质干细胞 在24 组织培养板中用MSC生长培养基接种间充质干细胞,每接种6x104个MSC(接种密度为3.15
底增加稳定性; 矩形:斜颈的矩形培养瓶瓶底至瓶颈是一个坡度设计,易于倾注,移液管或者细胞刮容易进入瓶体,大部分斜颈瓶都有一个裙边以增加稳定性。直颈和角度颈的矩形培养瓶整个瓶底都是培养面,节省空间并减少培养基因晃动而流到瓶盖。 以下是搜集的不同形状和用途的 细胞培养 瓶,主要包括:Nunc细胞培养瓶、Celstir夹套双侧臂细胞培养瓶、CellLine细胞培养瓶、Wheaton大容量细胞培养套装、美国 BELLCO 细胞悬浮培养瓶系列、美国 BELLCO
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