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500ml/
Lonza MSC GM-CD™ 00190632 BulletKit 间充质干细胞培养基
英文名称:TheraPEAK Chemically Defined Mesenchymal Stem Cell Growth Medium
| 产品应用: | 人类组织间充质干细胞培养 | ||
| 中国俗称: | 人间充质干细胞生长无血清培养基 | 中国简称: | 干细胞无血清培养基 |
| 注册品牌: | LONZA | 生产厂家: | LONZA美国 |
| 订购货号: | 00190632B | 保存温度: | 2℃~8℃,-10℃~-20℃ |
| 规格型号: | 500mL液体培养基和细胞因子 | ||
| 产品种类: | MSC间充质干细胞产品 | ||
产品英文名称:TheraPEAK Chemically Defined Mesenchymal Stem Cell Growth Medium
产品中文名称:人间充质干细胞生长无血清培养基
什么是间充质干细胞?
What’s the Mesenchymal Stem Cells or MSCs?
Mesenchymal stem cells, or MSCs, are multipotent stromal cells that can differentiate into a variety of cell types,including: osteoblasts (bone cells),chondrocytes (cartilage cells),and adipocytes (fat cells). This phenomenon has been documented in specific cells and tissues in living animals and their counterparts growing in tissue culture.Mesenchyme is embryonic connective tissue that is derived from the mesoderm and that differentiates into hematopoietic and connective tissue, whereas MSCs do not differentiate into hematopoietic cells.
关于人间充质干细胞生长无血清培养基
About TheraPEAK Chemically Defined Mesenchymal Stem Cell Growth Medium
TheraPEAK Chemically Defined Mesenchymal Stem Cell Growth Medium is a serum-free medium (SFM) specially formulated for the growth and expansion of human mesenchymal stem cells (MSCs). TheraPEAK MSCGM-CD SFM enables superior human MSC growth and increased consistency compared to classical serum-supplemented medium (DMEM + 10% FBS). Using TheraPEAK Chemically Defined Mesenchymal Stem Cell Growth Medium, human MSCs can be expanded beyond 5 passages while still maintaining their tri-lineage mesoderm differentiation potential (i.e., ability to differentiate into osteogenic, chondrogenic and adipogenic lineages).
In addition, TheraPEAK MSCGM-CD SFM facilitates expansion of MSCs directly from primary human bone marrow.
Lonza MSCGM-CD™ 00190632TheraPEAK Chemically Defined Mesenchymal Stem Cell Growth Medium 的保存条件
The preservation condition of TheraPEAK Chemically Defined Mesenchymal Stem Cell Growth Medium
Basal media:2℃~8℃(液体培养基储存在2℃~8℃)
SingleQuots Supplement: -10℃~-20℃(细胞因子添加物储存在-10℃~-20℃)
Lonza MSCGM-CD™ 00190632人间充质干细胞生长无血清培养基的优势
The advantage of TheraPEAK Chemically Defined Mesenchymal Stem Cell Growth Medium
◇Supports human mesenchymal stem cell growth(multi-potent adult stem cells) (支持人间充质干细胞的生长即多潜能成体干细胞)
◇No need for flasks coated with attachment matrix (不需要黏附基质包被细胞培养瓶)
◇Differentiation into osteobiasts, chondrocytes and adipocytes (可分化为:成骨细胞、软骨细胞和脂肪细胞)
◇Chemically defined and serum-free( 化学成分明确的无血清培养基)
◇For further manufacturing or laboratory use (可为进一步的生产或实验室研究使用)
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文献和实验Human Medulary thyroid tumor 人髓质甲状腺肿瘤细胞 ● U138 Human Glioma 人神经胶质瘤细胞 X-VIVO无血清培养基(X-VIVO Chemically Defined, Serum-free Hematopoietic Cell Media) 产品中文名称:LONZA X-VIVO 造血细胞无血清培养基 产品英文名称:LONZA X-VIVO™ 15 Chemically Defined, Serum
时,可选择TRUE1(TrueGel3D™水凝胶CD细胞可降解交联剂和RGD肽)作为起步配置。 试剂准备 按照产品说明书(datesheet或information sheet)准备试剂。 为了尽量减少巯基氧化,非必要时,勿将CD细胞可降解交联剂暴露于空气和室温。每次使用后务必迅速盖回试剂盖。 采用培养基、PBS或生理溶液配制储备细胞悬液或其他生物样本。 实验步骤 切记:开始混合试剂前,务必通读完整实验方案。 水凝胶混合液配制: 细胞悬液应占最终凝胶体积的1/5。相应地,配制完成凝胶的最终细胞
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
‐3 ligand, SCF, and TPO EGM2‐Bulletkit (to prepare ECFC expansion medium; Lonza, cat. no. CC‐3162) including: EBM
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