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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Clostridium botulinum Botulinum neurotoxin type A protein (1-436AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
HRP
- 适应物种:
Clostridium botulinum
- 保质期:
6个月
- 抗原来源:
Clostridium botulinum
- 目录编号:
P0DPI0
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA
- 浓度:
>95%,Protein G purified
- 靶点:
/
- 抗体英文名:
botA Antibody, HRP conjugated
- 抗体名:
botA;atx;bonT;Botulinum neurotoxin type A;BoNT/A;Bontoxilysin-A;BOTOX;Botulinum neurotoxin type A1)
- 规格:
100μg/50μg
| 规格: | 100μg | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥880.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
功能
Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
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胶原酶,收集筛网上子宫内膜腺体,经玻璃匀浆器匀浆后,1 000r/min离心20rain,弃沉淀,上清通过抗人全血清-Sepharose 4B柱,收集流洗液。浓缩后,双向免疫扩散与抗人全血清、抗人纤维蛋白原抗体之间应无沉淀线,作为包被抗原;②HRP标记的SPA结合物,用方阵法滴定最适工作浓度。 2.操作步骤 在聚苯乙烯反应板每孔加100uL子宫内膜抗原(1:100),4T:,包被过夜,常法洗涤。用含15%小牛血清的pH7.40.
祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
antibody unconjugated; 货号21337,产品描述:GAPDH Antibody;规格 50 ul / 100 ul; 货号3052,产品描述:Rabbit anti-Human IgG secondary antibody HRP conjugated; 货号300904050,产品描述:One-Hour Western kit,10 ml / bottle x 3;
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
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