Recombinant Serratia Marcescen

Using Electrostatics to Define the Active Site of Serratia Endonuclease

. Occasionally, a new protein structure is solved before the active site has been located through conventional methods. Using the extracellular endonuclease from Serratia marcescens as an example, we show here how the active site of an enzyme

粘质沙雷氏菌 serratia marcescens

亦称灵菌。一种产生鲜红色素的细菌。存在于空气和水中，可生长在动、植物性食品中。通常为0.7微米左右的近球形短杆菌，但形态多样。革兰氏阴性，有周生鞭毛。厌氧条件下也可以生长，但不产生色素。色素为灵菌红素（ prodigiosin），其生理作用不明。按 Bergey氏分类法，属于肠道细菌类。  

A Practical Guide for using the AdEasy System

for kanamycin resistance, and recombination confirmed by restriction endonuclease analyses. Finally, the linearized recombinant plasmid is transfected into adenovirus packaging cell lines, e.g. 293 cells. Recombinant adenoviruses are typically generated