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文献和实验上皮及间皮类常用标记抗体EPITHELIAL AND MESOTHELIAL MARKERSCK (MNF 116)- MNF-116 is a pan-cytokeratin antibody.- Source: Dakopatts.- Dilution 1:50.- Antigen retrieval method: HIER (pressure cooking) using EDTA.- Staining pattern: Cytoplasmic, typically
Fixation and permeabilization protocol 5/30/91 Mike Finney
are by themselves sufficient to open eggs. This technique should work for virtually all polyclonal sera most monoclonals, as long as the major epitopes do not depend on disulfide bonds. Animals fixed in this way can be stained with X-gal. 1) Fixation: - Wash animals
Preabsorbing Antisera with C. elegans Acetone Powder
Preabsorbing Antisera with C. elegans Acetone Powder by Michael Koelle, adapted from "Antibodies: A Practical Approach" by D. Catty One way to eliminate crossreactivity of a polyclonal antiserum raised against a worm
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