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- 克隆性:
单克隆
- 抗体英文名:
Anti-Wnt1
- 抗体名:
信号通路Wnt1抗体
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.
Also known as:
wingless-type MMTV integration site family, member 1; INT 1; INT1; Murine mammary tumor virus integration site 1 ; Oncogene INT1; Proto oncogene protein Wnt 1; Wnt 1; Wnt1 proto oncogene protein precursor
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文献和实验。HSP以直接或间接的方式参与了细胞凋亡的调节,其作用靶点涉及不同凋亡信号通路的各主要激酶或关键性调控蛋白。除对凋亡 相关信号转导通路的蛋白质直接调节作用之外,还涉及了一些细胞膜生长因子受体,如EGFR、ErbB2、FLT3及细胞内雌、雄激素受体等蛋白质稳定性和 活性的调节,这种调节作用具有双向性。即既有抗凋亡的作用,也有促凋亡的作用。细胞凋亡分为线粒体通路介导的凋亡及死亡受体通路介导的凋亡,而HSP不同 程度地对这两条通路的活化具有调节作用。Hsp90能过阴断Apaf-1寡聚化,最终阴断凋亡体的形成
免疫研究新方法:IGF信号通路抗体芯片筛选出肝癌靶生物标志物
单一片面的,之前因为缺少检测工具,无法对 IGF 信号通路中的蛋白表达进行全面的检测,本文第一次采用了RayBiotech(QAH-IGF-1)抗体芯片对 25 对肝癌与正常组织进行高通量检测对比。 IGF-2R 和 IGFBP-2 在肝癌组织中提高。其他 8 个没有显著变化。这为肝癌药物研究和开发提供新的靶标。
连接的标签有哪几种?标签是购买还是自己做?我想用lipo2000转染使wnt1导入目的细胞,逐步筛选等到稳定表达wnt1的细胞系可以吗? kinguangjun 你好!标签有很多种,最主要是要看你喜欢用什么标签,如His,FLAG等,标签是你在做克隆的时候加上去的,但Anti-His或Anti-FlAG的抗体要自己去购买的,如果载体上有抗生素的筛选标记,如Neomycin,Blasticidin等,就可以用抗生素去筛选稳转细胞株,如果没有的话,可能只能用慢病毒或逆转
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