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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Bcl-2-like protein 2 (BCL2L2)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Bcl-2-like protein 2 (BCL2L2)品名简称:
Recombinant Human BCL2L2 protein货号:
CSB-EP821707HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
BCL2L2Uniprot No.:
Q92843别名:
Apoptosis regulator Bcl-W种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
36.6 kDa表达区域:
2-193aa氨基酸序列:
ATPASAPDTRALVADFVGYKLRQKGYVCGAGPGEGPAADPLHQAMRAAGDEFETRFRRTFSDLAAQLHVTPGSAQQRFTQVSDELFQGGPNWGRLVAFFVFGAALCAESVNKEMEPLVGQVQEWMVAYLETQLADWIHSSGGWAEFTALYGDGALEEARRLREGNWASVRTVLTGAVALGALVTVGAFFASK蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.功能2:
Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.文献:
Bcl-w, a novel member of the Bcl-2 family, promotes cell survival.Gibson L., Holmgreen S.P., Huang D.C., Bernard O., Copeland N.G., Jenkins N.A., Sutherland G.R., Baker E., Adams J.M., Cory S.Oncogene 13:665-675(1996)研究领域:
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文献和实验Bcl-2蛋白是bcl-2 原癌基因的编码产物,是细胞存活促进因子,属膜整合蛋白,分子量为26kDa, 定位于线粒体、内质网和连续的核周膜。Bcl-2蛋白家族是一个特别的家族,成员中有些促进凋亡,如Bad、Bid 、Bax,有些成员阻止细胞凋亡,如Bcl-2、 Bcl-x、Bcl-w。Bcl-2能够阻止细胞色素c从线粒体释放到细胞质,从而抑制了细胞凋亡。
【求助】如果要证明Bcl-2和RARP活性,应该做什么实验?
eeflying 目前有两种化合物,分别为Bcl-2和PARP的抑制剂, 虽然这两个指标通常作为细胞凋亡的指标常用于分子生物实验, 但是,如何证明这两个分子被抑制的实验却第一次碰到, 请各位战友支招,我要直接检测哪个蛋白的WB 或者做什么实验合适? magichunter 用western检测bcl-2的含量变化,检测PARP活性裂解片段的含量变化以及原型态PARP的含量
bcl-2 原癌基因(bcl-2 protooncogene)
人的B-淋巴细胞(B-cell lymphomas)中染色体易位激活的原癌基因。bcl即是B-淋巴细胞的英文缩写。该基因编码一种细胞质膜蛋白,能够抑制细胞的程序性死亡。bcl-2原癌基因与秀丽隐杆线虫的ced-9基因同源。
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