THERMO植物直接PCR试剂盒

THERMO植物直接PCR试剂盒Phire Plant Direct PCR Kit
货号F130WH

描述

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant, fungi and bacteria.

The kit includes a complete set of optimized PCR reagents as well as detailed protocols for different sample types, making it an ideal choice for amplification of plant DNA. In addition, the kit includes control primers for positive control reactions.The Control Primers amplify a highly conserved region of chloroplast DNA and are universal for many plant species.

Highlights

• No need for time-consuming and expensive DNA purification steps
• Very little sample material required
• Two simple protocols for various applications
• Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb).

Applications

• Genotyping
• Transgene detection

内容及储存

Contains:
• 200 µL Phire Hot Start II DNA Polymerase
• 5 x 1.25 mL 2X Phire Plant Tissue PCR Buffer (includes dNTPs and MgCl₂)
• 40 µL Universal Control Primer mix (25 µM each)
• 2 x 5 mL Dilution Buffer

直接PCR—对植物、动物组织及血液样本直接扩增

hermo Scientific™ 直接PCR试剂

• 无需任何纯化步骤，只需微量的原始样本直接作为PCR反应的模板
• 已针对多种样本类型进行过测试
• 试剂盒组分完备——包括对照品、水和DNA分子量标准
• 类别

  	货号	产品名称	规格		数量
  预混液	F170S	Phire Tissue Direct PCR Master Mix	250 x 20 µL rxns
  	F170L	Phire Tissue Direct PCR Master Mix	1,250 x 20 µL rxns
  	F160S	Phire Plant Direct PCR Master Mix	250 x 20 µL rxns
  	F160L	Phire Plant Direct PCR Master Mix	1,250 x 20 µL rxns
  	F175S	Phusion Blood Direct PCR Master Mix	100 x 20 µL rxns
  	F175L	Phusion Blood Direct PCR Master Mix	500 x 20 µL rxns
  试剂盒	F140WH	Phire Animal Tissue Direct PCR Kit	500 reactions
  	F130WH	Phire Plant Direct PCR Kit	500 reactions
  	F150BID	Phusion Human Specimen Direct PCR Kit	200 reactions
  	F547S	Phusion Blood Direct PCR Kit	100 reactions
  	F547L	Phusion Blood Direct PCR Kit	500 reactions