- ￥5430.60
- StressMarq
- Canada
- SPC-314B-FITC
- 2025年07月13日
- WB, IP
- Rabbit
- The antibody is specific for salmonid Hsp70 (tested on salmon and brook trout). It does not cross react with Hsc70. It does not detect Hsp70 in other species.
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide conjugated to KLH. Peptide sequence specific to C-terminal part of salmonid Hsp70. The target is not present in any other Hsp70 proteins.
- 保存条件：
4°C
- 克隆性：
Polyclonal
- 标记物：
FITC
- 适应物种：
The antibody is specific for salmonid Hsp70 (tested on salmon and brook trout). It does not cross react with Hsc70. It does not detect Hsp70 in other species.
- 宿主：
Rabbit
- 应用范围：
WB, IP
- 抗体英文名：
Hsp70 (Fish) Antibody: FITC
- 规格：
200μg
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文献和实验for 5'. Gently slide off slip. 7. Labeling step: Add 100 µL of antibody (e.g. 1/400 avidin-FITC, Vector labs) diluted in blocking solution, Cover with large slip. Incubate r.t. x 30 min. 8. Wash step: Gently slide off cover slip. Wash
去除盖玻片； 6）43℃预热杂交后水洗溶液40ml水洗切片15min； 7）2×SSC(37℃)洗两次，每次10min； 8）切片放人染色缸的1×PBS内待检测，勿使切片干燥。 检测： 9）从1×PBS中取出切片，除去过多的水分，避免标本干燥。把切片放入湿盒内，同时处理4张切片。 10）每张切片使用30μl～60μl罗丹明抗-地高辛抗体或FITC卵白素，室温下孵育20min； 11）去掉塑料盖膜，把切片放入含1×PBS的染色缸。1×PBS室温下洗3次，每次2min
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
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