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Human
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文献和实验组测序,实现了人类基因组计划(Human Genome Project,HGP)中人类基因组的物理作图(physical mapping)和基因组测序相结合的目标。因此,这些克隆文库为基因定位研究提供了丰富的DNA 序列资源,NCBI Clone Registry、Ensembl Genome Browser和UCSC Genome Bioinformatics提供的网络生物信息学资源也记载了许多克隆基因组定位的详细信息,为我们选择相应克隆为作为染色体单一序列和端粒探针的来源提供了便利。
Intracellular Immunofluorescent Staining for Flow Cytometry
PBMCs were stimulated with TPA/ionomycin in the presence of monensin for 5 hours. The cells were fixed and permeabilized and intracellularly stained with anti-human CD4, clone RPA-T4, (Cat. No. 17-0049 ) and Alexa Fluor® 488 anti-human IL-17 (Cat. No. 53
Intracellular Immunofluorescent Staining for Flow Cytometry
PBMCs were stimulated with TPA/ionomycin in the presence of monensin for 5 hours. The cells were fixed and permeabilized and intracellularly stained with anti-human CD4, clone RPA-T4, (Cat. No. 17-0049 ) and Alexa Fluor® 488 anti-human IL-17 (Cat. No. 53
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