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- 详细信息
- 文献和实验
- 技术资料
- 宿主:
NA
- 适应物种:
Bovine;Human;Hamster;Mouse
- 克隆性:
多克隆
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文献和实验Antibody Validation by Western Blotting
by chemiluminescent, fluorescent, or colorimetric methods. This chapter focuses on the chemiluminescent detection of proteins using a manual western blotting system and a vacuum-enhanced detection system (SNAP i.d.™, Millipore)
Identifying Acetylated Proteins in Mitosis
outside of the transcriptional network has not been thoroughly assessed. We recently discovered that one of the HDACs, HDAC3, is localized on the mitotic spindle and regulates proper mitotic progression (1). To determine potential HDAC targets, we undertook a proteomics approach
antibodies, mAb):由一个识别一种抗原表位的B细胞 克隆产生的同源抗体。高度均一、特异性强、 效价高、少或无交叉反应性。 抗体的制备技术经历了三代: ™ 第一代 抗体 是传统的 抗体 制备方法即利用抗原免疫动物后获得抗体,称为多克隆抗体(polyclonal antibody); ™ 第二代抗体是通过杂交瘤技术制备出针对抗原中某一抗原决定簇的抗体称为单克隆抗体(monoclonal antibody, McAb
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