- ￥0 - 4000
- Merck Millipore
- 05-858
- 2025年08月19日
- NA
- Human;Chicken;Vertebrates;Yeast (<italic>S. cerevisiae</italic>)
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- 宿主：
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- 适应物种：
Human;Chicken;Vertebrates;Yeast (<italic>S. cerevisiae</italic>)
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
hyperphosphorylated H1 (pH1) from cells arrested in M phase by treatment with Colcemid (demecolcine; 0.06 µg/ml). The un‐ (Ac 0) and monoacetylated (Ac 1) isoforms of histone H4 are indicated. View Image
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