Anti-Dimethyl Histone H3 (Lys4

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure

Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides

that showed good Modulo staining throughout all the arms were analysed for loss of Polycomb binding. I strongly recommend using this anti-Modulo antibody as an internal control, rather than an antibody against an integral chromatin protein such as a histone

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications

by incubation in 100µl of elution buffer at 65°C for 5 minutes;he eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice