- ¥192
- 鼎国试剂
- 北京
- PER003-2
- 2025年11月26日
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- 库存：
99
- 供应商：
鼎国昌盛
- 规格：
1000u(2u/ul)
货号；PER003-2
规格;1000u(2u/ul)
价格;192元
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文献和实验Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye terminator reactions
One of the major problems in DNA cycle sequencing is that when fluorescent primers (1) are used the reaction conditions are such that the nested fragment set distribution is highly dependent upon the template concentration
, 150mM dATP, 150mM dCTP, 150mM c7deaza-dGTP and 15mM of the respective ddNTP) 5 x DNA labelling mix (10mM dGTP, 10mM dCTP, 10mM dTTP, 200mM Tris.Cl, pH 7.5, 250mM NaCl) 300mM DTT [a-35S]-dATP (~ 1000Ci/mmol, Amersham or DuPont) T7 DNA polymerase
A. Bst-catalyzed radiolabeled DNA sequencingBst DNA polymerase-catalyzed radiolabeled two-step sequencing reactions (26) are modified from those presented earlier (25) by altering the absolute amounts and the relative deoxy/dideoxynucleotide ratios
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