- 询价
- 1 kit
- 美国
- HH-14311D
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
南京赛泓瑞
- 规格：
1 ML
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文献和实验Dynabeads® Co-Immunoprecipitation Kit
and protein complexes are easily separated from lysate using magnetic separation properties of Dynabeads. Magnetic separation facilitates washing, buffer changes, and elution. Antibody-to-bead coupling works optimally with purified antibodies
Coupling Antibodies to Protein A or G
1. use 2 mg of antibody per ml wet beads (use appropriate antibody/protein A or G combination). 2. mix antibodies with beads and bind at room temperature for at least 1 hr (on roller). 3. wash the beads twice with 10 volumes borate buffer, spin
that need to be optimized, including the choice of antibody, the conditions for shearing chromatin using sonication and the analysis of immunoprecipitated DNA. The ChIP-IT Kit from Active Motif provides a complete solution to all potential problems associated with the ChIP
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