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H2N2 HA / Hemagglutinin抗体

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  • 询价
  • CST
  • 中国/美国/德国
  • hz-11688-RP02
  • 2025年07月09日
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    • 文献和实验
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      常温,避光

    • 克隆性

      单克隆

    • 抗体名

      H2N2 HA / Hemagglutinin抗体

    H2N2 HA / Hemagglutinin抗体产品信息

    免疫原 :
    Recombinant H2N2 (A/Canada/720/2005) HA / Hemagglutinin protein (Catalog#11688-V08H)
    Antibody Type : Rabbit Polyclonal Antibody ( Antibody Purification Platform )
    抗体宿主 :
    Rabbit IgG
    缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
    制备方法 :
    Produced in rabbits immunized with purified, recombinant H2N2 (A/Canada/720/2005) HA / Hemagglutinin (Catalog#11688-V08H; AAY28987.1; Met 1-Gln 516). H2N2 (A/Canada/720/2005) HA / Hemagglutinin specific IgG was purified by H2N2 (A/Canada/720/2005) HA / Hemagglutinin affinity chromatography.
    H2N2 HA / Hemagglutinin抗体Background
    Influenza (flu) is a respiratory infection in mammals and birds. This virus is divided into three main types (A, B and C). Influenza A is found in a wide variety of bird and mammal species and is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). H2N2 is a subtype of the species influenzavirus A. H2N2 has mutated into various strains including the Asian Flu strain (now extinct in the wild), H3N2, and various strains found in birds. It is also suspected of causing a human pandemic in 1889. Some believe that the 1889 - 1890 Russian flu was caused by the influenzavirus A virus subtype H2N2. Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. The HA is a trimer with a receptor binding pocket on the globular head of each monomer. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. Binding of HA to sialic acid-containing receptors on the surface of its target cell brings about the attachment of the virus particle to the cell and forms a endosome. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the hydrophobic portion "fusion peptide". After which, virus penetrates the cell and pours its contents including the RNA genome into the cytoplasm mediated by fusion of the endocytosed virus particle's own membrane and the endosomal membrane. Hemagglutinin plays a major role in the determination of host range restriction and virulence.
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    图标文献和实验
    相关实验
    • Anti-HA Immunocytology

        The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots against yeast protein

    • Immunocytology using the HA epitope

           The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots against yeast protein

    • 抗体的发展历程

      脾细胞与小鼠骨髓瘤细胞(P3-X63/Ag8)融合,融合的细胞既获得了亲代脾细胞分泌特异性抗体的特性,又具有骨髓瘤细胞大量繁殖的能力,成为一种既能分泌特异性抗体又能长命的杂交瘤细胞。该技术为抗体的分子生物学研究提供了全新的手段。极大地促进了免疫学,遗传学,分子生物学的快速发展。   但传统抗体目前也面临诸多问题,如: 每使用一管新抗体都需要进行滴定测试; 多种同型对照,实验设计繁琐; 需要添加Fc阻断试剂; 抗体不佳,阳性细胞群不明显; 抗体作为细胞生物学和生物化学中最重要的试剂之一,科学界每年在这类

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