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- 文献和实验
- 技术资料
- 保存条件:
常温,避光
- 克隆性:
单克隆
- 抗体名:
14-3-3 beta / YWHAB抗体
免疫原 :
Recombinant Human 14-3-3 beta / YWHAB protein (Catalog#10843-H09E)
Antibody Type : Rabbit Polyclonal Antibody ( Antibody Purification Platform )
抗体宿主 :
Rabbit IgG
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 :
Produced in rabbits immunized with purified, recombinant Human 14-3-3 beta / YWHAB (rh 14-3-3 beta / YWHAB; Catalog#10843-H09E; NP_003395.1; Met1-Asn246). 14-3-3 beta / YWHAB specific IgG was purified by Human 14-3-3 beta / YWHAB affinity chromatography.
14-3-3 beta / YWHAB抗体Background
Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB), also known as14-3-3 protein beta / alpha and protein kinase C inhibitor protein 1 (KCIP1), is a member of the 14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAB has been repoted to interact with SSH1,TORC2/CRTC2, ABL1, ROR2, GAB2, and thus is invloved in the related processes, such as apoptosis. Inaddition, YWHAB may serve as a negative regulator of osteogenesis.
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文献和实验的关系理不出头绪。GSK-3 beta 总蛋白减少,p-GSK-3 beta (ser 9)增加,是不是足以说明有活性的GSK-3 beta 减少更多,这样理解不知道对不对,希望给以指点。 galaxy22 楼主你好,请问你做出结果的tyr216的抗体是哪个公司的。。。我做出来总量是减少的,active B catenin是减少的,那我有必要做ser9 和tyr216的磷酸化形式吗?我想研究wnt和akt。。。 棉花糖的温柔
familar 小妹困惑,在此请教大师! 做了几次beta-catenin的表达(western),都没做出来,目的蛋白的分子量是92,可是,做出来的印记总是在下方,明显的不是目的蛋白的位置。我师兄说,做beta-catenin要提核蛋白才能做出来,可我之前也有做出来过的!请教各位有经验的同志,这样的现象是何解? woxingwosu 不一定要提核蛋白才能出来。b-catenin在核内核外都有分布。应该
Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
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