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- 文献和实验
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大量
- 供应商:
西宝生物
- 规格:
250ML
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文献和实验can be embedded in paraffin for sectioning and conventional histological staining. Immerse slide into xylene to remove mounting medium Change xylene 2 times Transfer to 1:1 xylene:paraffin (60°C) Change xylene 2 times and embed in paraffin
Embedding, Sectioning, and Mounting of Frozen Tissues
tissue-side of the slide is still visibly wet. Lay the slide flat on the paper towel, and apply two drops of Crystal/Mount to the face of the slide opposite the labelside. apply 2 drops here Immediately apply the 24x60 mm coverslip, left edge
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBR
may be used. Try to avoid having more than a monolayer of embryos (tilt vial to increase surface area). Devitillenize with a methanol shock. Remove the aqueous (bottom = fix) phase completely (use a drawn out pipet) as well as one pipetful of heptane
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