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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
DNA Clean & Concentrator-500
- 供应商：
简石生物
HIGHLIGHTS
- Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease digestions, and cell-free DNA preps, etc.
- Unique column construction allows sample loading and washing to be performed using a centrifuge, microcentrifuge, or vacuum source.
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
DESCRIPTION
The DNA Clean & Concentrator-500 (DCC-500) is our highest capacity PCR purification kit of the DNA Clean & Concentrator products. It is designed for the rapid, large format purification and concentration of up to 500 µg of high quality DNA from samples such as large-scale restriction endonuclease digestions and crude DNA preparations. Eluted DNA is well suited for use in PCR, DNA sequencing, DNA transfection, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc. The entire DNA purification/concentration procedure typically takes less than 20 minutes.
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文献和实验3 times with 500μl wash solution opellet silica once more to remove any residual wash solution oair dry to remove any residual EtOH 3.Elute DNA from silica oresuspend silica pellet in desired volume of 10 mM Tris 7.5 or TE or 1X restriction buffer
Clean-up Spin Protocol For DNA fragments (30bp-100 bp) 1) Add 3 x volume of buffer P3, 3 x volume of isopropanol to the enzymatic reaction and mix throughly with pipetting or vortex. The maximum volume of the reaction can be processed
(10 seconds in 'nanofuge' or brief spin at full spend in microfuge) wash silica 3 times with 500 ul wash solution pellet silica once more to remove any residual wash solution air dry to remove any residual EtOH 3.Elute DNA from silica
