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- 保存条件:
常温,避光
- 克隆性:
单克隆
- 抗体名:
Cystatin E / CST6抗体
免疫原 :
Recombinant Human Cystatin E / CST6 protein (Catalog#10438-H08H)
Antibody Type : Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
克隆号 :
034
抗体宿主 :
Rabbit IgG
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 :
This antibody was obtained from a rabbit immunized with purified, recombinant Human Cystatin E / CST6 (rh Cystatin E / CST6; Catalog#10438-H08H; NP_001314.1; Met 1-Met 149).
Cystatin E / CST6抗体 Background
Cystatin E/M, also referred to as CST6, is a member of type 2 cysteine proteinase inhibitors of the cystatin superfamily, and inhibits papain and cathepsin B. Cystatin E is a low molecular mass secreted protein existing in both a glycosylated (17 kDa) and an unglycosylated (14 kDa) form, with two characteristic intrachain disulfide bridges. Expression of cystatin M/E is found to be restricted to the epidermis, more specifically in the stratum granulosum, sweat glands, sebaceous glands, and the hair follicles. In addition to its function as a cysteine protease inhibitor, cystatin M/E also serves as a target for cross-linking by transglutaminases. Accordingly, cystatin M/E was suggested to be involved in barrier formation and maintenance. Furthermore, studies have revealed that cystatin M/E is frequently epigenetically inactivated during breast carcinogenesis, and thus be regarded as a candidate of tumour suppressor gene.
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文献和实验E-Z 96 X-press Plasmid Vacuum Protocol
well plate inside the manifold base; 3) Place the top plate of manifold over the base, the deep well plate or waste collection tray now should be positioned under the E-Z 96 X-press Plate. Seal the unused wells of E-Z 96 Xpress Plate with sealing
E-Z 96 X-press Plasmid Spin Protocol
-well plate. Transfer the cell lysate to each well of E-Z 96 X-press plate. 3. Centrifuge at 3000 x g for 5 minutes at room temperature. Discard the flow-through and re-use the deep well plate. 4. Add 500μl DLW Buffer (diluted with isopropanol
E. Z. N.A.TM X-press Plasmid Protocol
实验试剂 Materials to Be Provided by User 1. Ice 2. Isopropanol (100%) 实验设备 Materials to Be Provided by User 1. Tabletop micro-centrifuge capable of 13,000 x g 2. Nuclease Free
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