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文献和实验Isolation of c-Kit+ Human Amniotic Fluid Stem Cells from Second Trimester
to obtain c-Kit+ human AFS cells, starting from second trimester amniocentesis samples.
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
. Cell Sorting (Lin-,cKit+,Sca1+): 1. Incubate cells in FcgRII block on ice for 10 min. 2. Stain an aliquot and with PE-IgG2a and FITC-IgG2b isotype antibodies as neg. controls. Incubate remainder of cells with Sca-1 PE, plus c-kit FITC antibodies
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