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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
PE anti-human CD117 (c-kit)
- 抗体英文名:
PE anti-human CD117 (c-kit)
- 供应商:
上海善然生物科技有限公司
- 保存条件:
2-8℃
- 规格:
100tests
PE anti-human CD117 (c-kit) Antibody
W18195C
Catalog# / Size
375306 / 100 tests
Clone
Regulatory Status RUO
Other Names Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
Isotype Rat IgG2a, κ
Description CD117 is a 145 kD protein tyrosine kinase also known as c-kit. It is a receptor for stem cell
factor or c-kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells
(approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML).
CD117 binding of c-kit ligand induces phosphorylation of CD117 and stimulates proliferation
and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulomonocytic committed cells.
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文献和实验Isolation of c-Kit+ Human Amniotic Fluid Stem Cells from Second Trimester
to obtain c-Kit+ human AFS cells, starting from second trimester amniocentesis samples.
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
. Cell Sorting (Lin-,cKit+,Sca1+): 1. Incubate cells in FcgRII block on ice for 10 min. 2. Stain an aliquot and with PE-IgG2a and FITC-IgG2b isotype antibodies as neg. controls. Incubate remainder of cells with Sca-1 PE, plus c-kit FITC antibodies
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