相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 亚型:
1gG
- 保存条件:
2-8℃ 短期保存或<-20℃长期保存保存1年以上
- 克隆性:
多克隆
- 标记物:
电询
- 适应物种:
hu, mo, rat
- 宿主:
电询
- 应用范围:
电询
- 浓度:
1mg/1ml
- 靶点:
电询
- 抗体英文名:
GFP
- 抗体名:
绿色荧光蛋白
- 规格:
0.5mg1mg
中文名称:绿色荧光蛋白
英文名称:GFP
货号:ym-c0639P
规格:0.5mg1mg
级别:分子生物学级, 亲和纯化
抗原:多肽抗原
抗原来源:多肽合成
抗体来源:Rabbit
适用物种:hu, mo, rat
可提供抗体IgG的各种标记conjugate:可提供抗体IgG的各种标记 Isotype:可咨询
应用范围:WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
克隆类型:Polyclonalormonoclonal
交叉反应:Human,Mouse,Rat,Dog,Horse
保存环境:2-8℃ 短期保存或<-20℃长期保存保存1年以上,避免反复冻融。
绿色荧光蛋白Rules:
(1) the initial reaction to produce antibodies to antigens: when the first to enter the body, need to produce antibodies after certain incubation period can, and antibody production quantity are not many, in the body to maintain the time is short.
(2) once again: when the same antigen antibody reaction to produce second times into the body, at the beginning, as part of the original antibody binding and once again into the antigen antibody, can make the original amount reduced slightly. Subsequently, rapid increase antibody titer, several times more than the initial reaction can be generated to a few times, retained in the body of a lot of time.
(3) recall response to produce antibodies: antibodies produced by antigen stimulation of the body, after a certain period of time can disappear gradually. If the re exposure to antigen, the antibody has faded fast rise. As the body with the same antigen again first, then known as the specific recall responses; and if the initial reactions of different, it is known as a nonspecific recall response. Increased nonspecific memories to elicit antibody responses are temporary, short time soon fall.
绿色荧光蛋白相关产品:
ZNF268 (zinc finger protein ZNF268)- N-terminal (1a)锌指脂蛋白-1a(抗原)ym-c0625P
VSIG4 (V-set and immunoglobulin domain-containing protein 4)VSIG4 T淋巴细胞负调节蛋白之一(抗原)ym-c0479P
ZNF300 (zinc finger protein ZNF300)- middle锌指蛋白300(抗原)ym-c0628P
VAP1(vascular adhesion protein 1)血管粘附蛋白1(多肽抗原)ym-c0853P
Tyrosinase酪氨酸酶(多肽抗原)ym-c0819P
TSLP(Thymic stromal lymphopoietin isoform 1)淋巴细胞生成素-1(抗原)ym-c0449P
SYK (Spleen tyrosine kinase)非受体型酪氨酸激酶(抗原)ym-c0685P
SP-A (pulmonary surfactant-associated glycoprotein A)肺表面活性蛋白A(抗原)ym-c0770P
RRAD (Ras-related associated with diabetes )RRAD(多肽抗原)ym-c0278P
p21/WAF1/CIP1 p21 核分裂抑制因子之一(抗原)ym-c0741P
galectin 9 半乳糖凝集素9(抗原)ym-c0604P
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验一、 GFP背景概述 绿色荧光蛋白(green fluorescent protein, GFP)最早由Osamu Shimomura于1962年在水母(Aequorea victoria)(图2a)中发现。这种蛋白质在蓝色波长范围的光照激发下发出绿色荧光,其发光过程需要冷光蛋白质Aequorin的帮助,而且,这个冷光蛋白质可与钙离子(Ca2+)相互作用(图3)。在Aequorea victoria中发现的野生型绿色荧光蛋白的分子量较小,仅为27~30kDa,而编码GFP的基因序列也很短
dmdfe 本人将GFP基因后面加了一段大约110bp的序列,插入PET28A的BamH1和Hind3位点,最后在BL21中表达。 经SDS-PAGE验证表明: 目的蛋白 大量存在菌体裂解后沉淀即包涵体中,但是该沉淀无色。 少量存在于上清中,上清显微弱的绿色。 看文献大量报道GFP融合蛋白几乎都形成可溶性表达,目的蛋白在上清中,并且诱导条件没有太大要求。 想请教一下各位做过GFP原核表达的经验
刚刚去BD网站,发现一个优惠活动,送BD绿色荧光蛋白表达载体,原价是6000千元的,赶快贴在这里,大家赶快去要呀,是个好东东!! Free AcGFP green monomer vector while supplies last! Commemorative Promotion: Ten Years in Fluorescent Proteins Dear Researchers, Over the past ten years, your feedback
技术资料暂无技术资料 索取技术资料





