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文献和实验Recombineering/Lambda red-mediated gene replacement
Overview Single-gene knockouts using λ red system, adapted from Datsenko and Wanner paper. The goal of this protocol was to create an endA (endonuclease I) knockout, but obviously it can be adapted to any gene. The knocked-out gene
Directed Differentiation of Red Blood Cells from Human Embryonic Stem Cells
banks of hESC lines with matched or reduced incompatibility could potentially reduce or eliminate the need for immunosuppressive drugs and/or immunomodulatory protocols altogether, for example, O-type RhD− lines for generation of universal red blood
A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay
In order to determine the activity of O-linked GlcNAc transferase (OGT), a modified coupled enzyme method was proposed. This method was based on the measurement of uridine 5′-(trihydrogen diphosphate) (UDP), a product generated
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