- ￥3136
- 吉满/Genomeditech
- USA
- GM-021002
- 2025年12月24日
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现货质粒/干扰质粒/过表达质粒
￥1599
Hmgn5 Mus musculus high-mobility group nucleosome binding domain 5 (Hmgn5), mRNA.
询价
Cd24 Rattus norvegicus CD24 molecule (Cd24), mRNA.
询价
MGRN1 Homo sapiens mahogunin ring finger 1 (MGRN1), transcript variant 2, mRNA.
询价Chd6 Mus musculus chromodomain helicase DNA binding protein 6 (Chd6), mRNA.
询价
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 英文名：
VDR(VitaminD Receptor)
- 库存：
1ug
- 供应商：
吉满/Genomeditech
- 规格：
1μg
pGMVDR-Lu是吉满生物改造后的哺乳动物真核表达载体，在其多克隆位点插入了多个VDR结合位点，可以高灵敏度地检测VDR的激活水平。同时，对载体中预测出的其它转录因子以外的结合位点进行了适当的突变，增加了质粒的转录因子结合特异性。另外，由于质粒体积减小，使得VDR报告基因质粒更易于转染。
质粒图谱：
| VDR response element (VDR) | 32-61 |
| Minimal TA promoter (pTA) | 90-112 |
| Luciferase reporter gene | 144-1806 |
| SV40 late poly(A) signal | 1841-2062 |
| SV40 early promoter | 2110-2528 |
| Synthetic neomycin phosphotransferase(Neor) coding region | 2553-3347 |
| Synthetic poly(A) signal | 3372-3420 |
| Synthetic Beta-lactamase(Ampr) coding region | 4535-5395 |
| Synthetic poly(A) signal/transcriptional pause site | 5500-5653 |
| 产品编号 | 产品名称 | 包装 |
| GM-021002 | VDR报告基因 | 1μg |
| - | 说明书 | - |
pGMVDR-Lu可以采用常规转染方法转染哺乳动物细胞。用荧光素酶检测试剂盒或双荧光素酶检测试剂盒进行检测。
注意事项：
本质粒未经吉满生物允许不得用于任何商业用途，也不得移交给订货人实验室以外的任何人或单位。
为了您的健康，实验操作时请穿实验服和带一次性手套。
保存条件：
-20℃保存。
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文献和实验[2] Choi M, Ozeki J, Hashizume M, Kato S, Ishihara H, Makishima M.Vitamin D receptor activation induces peptide YY transcription in pancreatic islets.Endocrinology. 2012 Nov;153(11):5188-99. doi: 10.1210/en.2012-1396. Epub 2012 Sep 7.
[3] Gupta GK, Agrawal T, Del Core MG, Hunter WJ 3rd, Agrawal DK.Decreased expression of vitamin D receptors in neointimal lesions following coronary artery angioplasty in atherosclerotic swine.PLoS One. 2012;7(8):e42789. doi: 10.1371/journal.pone.0042789. Epub 2012 Aug 6.
Plasmid-Based Reporter Genes: Assays for Green Fluorescent Protein
useful as a generic tag for studying protein synthesis, translocation, and other protein–protein interactions. GFP is also widely used as a reporter in many genetic techniques, including transposon mutagenesis, promoter/enhancer traps, and one-component
Plasmid-Based Reporter Genes: Assays for -Galactosidase and Alkaline Phosphatase Activities
Reporter genes encode easily measurable traits. Most commonly, they are used to investigate the expression of other genes for which functional assays are not available or for which measurement of expressed product is difficult
Reporter genes have been widely used in plant molecular biology, typically to discern patterns of gene expression, but also as markers of transformed cells during stable transformation procedures. The ideal marker gene
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