- ¥3484
- BD
- 558264
- 2025年08月28日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
多克隆
- 抗体英文名：
Human Soluble Protein Master Buffer Kit
- 抗体名：
流式检测Buffer Kit
- 规格：
100 Tests
BD Cytometric Bead Array (CBA)
Contents
Assay diluent, capture bead diluent, capture bead diluent for serum/plasma samples, detection reagent diluent, wash buffer, instrument setup reagents
Application
Flow cytometry (Routinely Tested)
The BD™ CBA Human Soluble Protein Flex Set System employs particles with discrete fluorescence intensities to detect soluble analytes at very low concentrations. The working assay range for most analytes in this system is 10 to 2,500 pg/mL.
The BD™ CBA Human Soluble Protein Master Buffer Kit contains all of the supporting reagents necessary to perform an assay using a BD CBA Human Soluble Protein Flex Set. The buffers and instrument setup reagents provided in this kit have been optimized for analysis of analytes in tissue culture supernatants, plasma, and serum samples. The BD CBA Human Soluble Protein Master Buffer Kit provides sufficient reagents for the quantitative analysis of 100 samples and 10 instrument setup procedures (Catalog No. 558264) or 500 samples and 10 instrument setup procedures (Catalog No. 558265).
| Component | Quantity (558264) | Quantity (558265) |
| Assay Diluent | 1 bottle, 30 mL | 1 bottle, 150 mL |
| Capture Bead Diluent | 1 bottle, 5 mL | 1 bottle, 30 mL |
| Detection Reagent Diluent | 1 bottle, 5 mL | 1 bottle, 30 mL |
| Capture Bead Diluent for Serum/Plasma | 1 bottle, 5 mL | 1 bottle, 30 mL |
| Wash Buffer | 1 bottle, 130 mL | 1 bottle, 650 mL |
| Instrument Setup Bead A1 | 1 vial, 0.25 mL | 1 vial, 0.25 mL |
| Instrument Setup Bead A9 | 1 vial, 0.25 mL | 1 vial, 0.25 mL |
| Instrument Setup Bead F1 | 1 vial, 1.0 mL | 1 vial, 1.0 mL |
| Instrument Setup Bead F9 | 1 vial, 0.25 mL | 1 vial, 0.25 mL |
| PE Instrument Setup Bead F1 | 1 vial, 0.25 mL | 1 vial, 0.25 mL |
| PE Positive Control Detector | 1 vial, 0.5 mL | 1 vial, 0.5 mL |
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文献和实验Dynabeads® Co-Immunoprecipitation Kit
Co-Immunoprecipitation Optimization below) from which the desired protein complex is isolated. The Extraction Buffer is made with 5 × IP supplied in the kit. The 5 × IP is supplied as 5 × concentrate and in sufficient quantity to allow for 40 coimmunoprecipitation
Native Chromatin Preparation and Illumina/Solexa Library Construction
) (see Step 39) The kit includes end-repair enzyme mix, end-repair 10X buffer, dNTP solution, and ATP. Dynabeads Protein A (Invitrogen) Ethanol (70% and 100%) Glycerol Glycogen, 20 mg/mL Klenow fragment exo- (optional; see Step
CellTrace CFSE Cell Proliferation Kit
or other suitable buffer. Standard fixation protocols using aldehyde-containing fixatives should effectively crosslink the amines of the protein–probe conjugate. Typically, cells are fixed for 15 min at room temperature using 3.7% formaldehyde.
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