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文献和实验Sample Solublization Buffers for Two-Dimensional Electrophoresis
urea and 2 M thiourea), 2–4% nonionic and/or zwitterionic detergent(s), reducing agent(s), carrier ampholytes and, depending on the type of sample, protease inhibitors. In this chapter, the major constituents of sample solubilization/lysis buffers
Immunoprecipitation of E1 A-Containing Protein Complexes
Important insights into cell growth and transcriptional regulation have been realized by correlating adenovirus ElA-induced cell-growth-control mechanisms with ElA’s specific interactions with host-cell proteins (see ref. 4 ). A key method
Introduction to the EMSA (Gel Shift) Technique
(such as transcription factors) in crude lysates and, in conjunction with mutagenesis, to identify the important binding sequences within a given gene’s upstream regulatory region. EMSAs can also be utilized quantitatively to measure thermodynamic and kinetic parameters
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