- ￥3560.01
- Qiagen
- Made in Germany
- 205111
- 2026年03月29日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
Room Temperature
- 保质期：
1年
- 英文名：
Omniscript RT Kit
- 库存：
10⁺盒
- 供应商：
上海睿安生物19121878899
- 规格：
50次/盒
Qiagen货号205111反转录试剂盒Omniscript RT Kit上海睿安生物19121878899
Features
- High cDNA yields due to high-affinity enzyme
- Sensitive detection of as few as 10 copies of template
- No additional RNase H digestion step required
- Fast and easy procedure with no tedious pipetting steps
Product Details
Performance
The high affinity of Omniscript Reverse Transcriptase (RT) results in highly specific and sensitive RT-PCR, even with low-copy transcripts (see figure "
Sensitive RT-PCR of ≥10 copies.
Regions of RNA with high GC content can cause other reverse transcriptases to stop, dissociate from the RNA template, or skip over looped-out regions of RNA (see figure " Full-length RT-PCR products — B"). These difficult templates, however, prove no problem for QIAGEN reverse transcriptases (see figure " Full-length RT-PCR products — A"). With no need for optimization, the Omniscript RT Kit makes reverse transcription virtually trouble-free.
Principle
Omniscript Reverse Transcriptase (RT) has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, leading to high yields of cDNA. It is provided ready-to-use with dNTPs and in an optimized reaction buffer that, together with the high affinity of Omniscript RT for RNA, enables read-through of templates with high GC content or complex secondary structure. Please note that the primer mix is not provided.
Omniscript RT is specially designed for all reverse transcription with any amount of RNA from 50 ng to 2 µg per reaction. Omniscript RT is also usually the enzyme of choice with viral RNA due to the presence of carrier RNA in most viral RNA preparations. In comparative experiments, Omniscript RT consistently outperforms other reverse transcriptases over a wide range of starting RNA amounts.
Lot-to-lot reproducibility of Omniscript Kits is ensured by rigorous quality control at QIAGEN. The optimized Buffer RT, dNTPs, and water included in all Omniscript RT Kits are guaranteed RNase-free, and each lot of Omniscript RT is thoroughly tested for RT-PCR reproducibility.
Procedure
Applications
Omniscript Reverse Transcriptase is suitable for use in the following applications:
- cDNA synthesis for end-point PCR
- Synthesis of double-stranded cDNA for cloning
- RACE (Rapid Amplification of cDNA Ends)
- Linear RNA amplification
- Exponential RNA amplification
- SAGE (Serial Analysis of Gene Expression)
- Labeling for microarrays
- Analysis of transcription start site by primer extension
QIAGEN是全球领先的样本和分析技术的供应者，提供各种样本纯化和分析的方法和产品。先进的、高质量的产品和服务确保了从样本获得分析结果。
·快速合成cDNA，进行两步法real-time RT-PCR，用于基因表达分析
·cDNA合成和gDNA去除仅用20分钟完成
·即便是低丰度的转录本也能获得高产量的cDNA
·转录本的5'和3'端区域都可以进行cDNA合成
·无需设计RNA特异性引物或探针
通过方便快速的操作过程进行cDNA合成，并有效去除基因组DNA。应用gDNA Wipeout Buffer可高效去除RNA样本中的基因组DNA污染。QuantiTect Reverse Transcription Kit提供所有快速、高效逆转录所需的组分，包括Quantiscript Reverse Transcriptase、Quantiscript RT Buffer和独特的RT Primer Mix。合成的cDNA经过优化可用于real-time PCR，实现对mRNA转录本所有区域的靶分子进行可靠的定量检测。
订购信息:
| 货号 | 产品名称 | 产品说明 |
| 69506 | DNeasy Blood & Tissue Kit (250) | 血液，组织DNA提取试剂盒 |
| 69504 | DNeasy Blood & Tissue Kit (50) | 血液，组织DNA提取试剂盒 |
| 69104 | DNeasy Plant Mini Kit (50) | 从植物细胞和组织或真菌样本中纯化总DNA |
| 12362 | EndoFree Plasmid Maxi Kit (10) | 质粒大提试剂盒 |
| 12381 | EndoFree Plasmid Mega Kit (5) | 质粒大提试剂盒 |
| 59104 | EpiTect Bisulfite Kit (48) | 甲基化转化试剂盒 |
| 28606 | MinElute Gel Extraction Kit (250) | 胶体DNA回收试剂盒 |
| 217004 | miRNeasy Mini Kit (50) | microRNA提取试剂盒 |
| 30210 | Ni-NTA Agarose (25 ml) | 6His蛋白纯化柱料 |
| 205111 | Omniscript RT Kit (50) | 反转录试剂盒 |
| 51106 | QIAamp DNA Blood Mini Kit (250) | 血液DNA提取试剂盒 |
| 51104 | QIAamp DNA Blood Mini Kit (50) | 血液DNA提取试剂盒 |
| 51304 | QIAamp DNA Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 51504 | QIAamp DNA Stool Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 20021 | QIAEX II Gel Extraction Kit (150) | 胶回收纯化DNA |
| 12143 | QIAGEN Plasmid Midi Kit (25) | 质粒中提试剂盒 |
| 27104 | QIAprep Spin Miniprep Kit (50) | 提取高纯度质粒DNA |
| 28704 | QIAquick Gel Extraction Kit (50) | 胶回收试剂盒 |
| 28104 | QIAquick PCR Purification Kit (50) | PCR回收试剂盒 |
| 37900 | Qproteome Bacterial Protein Prep Kit | 细菌蛋白制备试剂盒 |
| 76106 | RNAlater (250ml) | RNAlater保护液 |
| 76104 | RNAlater (50ml) | RNAlater保护液 |
| 79254 | RNase-Free DNase Set (50) | DNA降解酶 |
| 74106 | RNeasy Mini Kit (250) | 细胞组织RNA提取试剂盒 |
| 74104 | RNeasy Mini Kit (50) | 细胞组织RNA提取试剂盒 |
| 74904 | RNeasy Plant Mini Kit (50) | 细胞组织RNA提取试剂盒 |
睿安公司专业技术服务
荧光定量PCR、甲基化检测、文库构建、原核和真核蛋白表达、高通量测序、抗体定制服务、WB检测、IHC检测。
睿安公司代理及优势品牌：
免疫学：Abgent、CST、R&D、Abcam、BD、Epitomics、Santa cruz、Jackson
耗材类： Ammex、Fisher、Bioplastics、Axygen、Corning、Greiner，Nunc-Nalgene
分子和细胞：Fermentas、hyclone-pierce、Qiagen、Bio-Rad
综合类： Sigma、 MERCK、Millipore、Roche、Invitrogen
自主品牌：Rainbio 生化类：Sigma-Vetec 、 MP
肥胖代谢类：Biovendor、ALPCO
表观遗传：Zymo ACTIVE MOTIF 神经和信号传导：Tocris
其他类： Biovision、Biocolor、Covance、Cytoskeleton、ebioscience、GE、Genetex、Invivogen、Omega、
Proteintech 、Socorex 、Vector
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- 作者
- 内容
- 询问日期
文献和实验Qiagen货号205111反转录试剂盒Omniscript RT Kit上海睿安生物19121878899
Features
- High cDNA yields due to high-affinity enzyme
- Sensitive detection of as few as 10 copies of template
- No additional RNase H digestion step required
- Fast and easy procedure with no tedious pipetting steps
Product Details
Performance
The high affinity of Omniscript Reverse Transcriptase (RT) results in highly specific and sensitive RT-PCR, even with low-copy transcripts (see figure "
Sensitive RT-PCR of ≥10 copies.
Regions of RNA with high GC content can cause other reverse transcriptases to stop, dissociate from the RNA template, or skip over looped-out regions of RNA (see figure " Full-length RT-PCR products — B"). These difficult templates, however, prove no problem for QIAGEN reverse transcriptases (see figure " Full-length RT-PCR products — A"). With no need for optimization, the Omniscript RT Kit makes reverse transcription virtually trouble-free.
Principle
Omniscript Reverse Transcriptase (RT) has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, leading to high yields of cDNA. It is provided ready-to-use with dNTPs and in an optimized reaction buffer that, together with the high affinity of Omniscript RT for RNA, enables read-through of templates with high GC content or complex secondary structure. Please note that the primer mix is not provided.
Omniscript RT is specially designed for all reverse transcription with any amount of RNA from 50 ng to 2 µg per reaction. Omniscript RT is also usually the enzyme of choice with viral RNA due to the presence of carrier RNA in most viral RNA preparations. In comparative experiments, Omniscript RT consistently outperforms other reverse transcriptases over a wide range of starting RNA amounts.
Lot-to-lot reproducibility of Omniscript Kits is ensured by rigorous quality control at QIAGEN. The optimized Buffer RT, dNTPs, and water included in all Omniscript RT Kits are guaranteed RNase-free, and each lot of Omniscript RT is thoroughly tested for RT-PCR reproducibility.
Procedure
Applications
Omniscript Reverse Transcriptase is suitable for use in the following applications:
- cDNA synthesis for end-point PCR
- Synthesis of double-stranded cDNA for cloning
- RACE (Rapid Amplification of cDNA Ends)
- Linear RNA amplification
- Exponential RNA amplification
- SAGE (Serial Analysis of Gene Expression)
- Labeling for microarrays
- Analysis of transcription start site by primer extension
QIAGEN是全球领先的样本和分析技术的供应者，提供各种样本纯化和分析的方法和产品。先进的、高质量的产品和服务确保了从样本获得分析结果。
·快速合成cDNA，进行两步法real-time RT-PCR，用于基因表达分析
·cDNA合成和gDNA去除仅用20分钟完成
·即便是低丰度的转录本也能获得高产量的cDNA
·转录本的5'和3'端区域都可以进行cDNA合成
·无需设计RNA特异性引物或探针
通过方便快速的操作过程进行cDNA合成，并有效去除基因组DNA。应用gDNA Wipeout Buffer可高效去除RNA样本中的基因组DNA污染。QuantiTect Reverse Transcription Kit提供所有快速、高效逆转录所需的组分，包括Quantiscript Reverse Transcriptase、Quantiscript RT Buffer和独特的RT Primer Mix。合成的cDNA经过优化可用于real-time PCR，实现对mRNA转录本所有区域的靶分子进行可靠的定量检测。
订购信息:
| 货号 | 产品名称 | 产品说明 |
| 69506 | DNeasy Blood & Tissue Kit (250) | 血液，组织DNA提取试剂盒 |
| 69504 | DNeasy Blood & Tissue Kit (50) | 血液，组织DNA提取试剂盒 |
| 69104 | DNeasy Plant Mini Kit (50) | 从植物细胞和组织或真菌样本中纯化总DNA |
| 12362 | EndoFree Plasmid Maxi Kit (10) | 质粒大提试剂盒 |
| 12381 | EndoFree Plasmid Mega Kit (5) | 质粒大提试剂盒 |
| 59104 | EpiTect Bisulfite Kit (48) | 甲基化转化试剂盒 |
| 28606 | MinElute Gel Extraction Kit (250) | 胶体DNA回收试剂盒 |
| 217004 | miRNeasy Mini Kit (50) | microRNA提取试剂盒 |
| 30210 | Ni-NTA Agarose (25 ml) | 6His蛋白纯化柱料 |
| 205111 | Omniscript RT Kit (50) | 反转录试剂盒 |
| 51106 | QIAamp DNA Blood Mini Kit (250) | 血液DNA提取试剂盒 |
| 51104 | QIAamp DNA Blood Mini Kit (50) | 血液DNA提取试剂盒 |
| 51304 | QIAamp DNA Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 51504 | QIAamp DNA Stool Mini Kit (50) | 人类组织和细胞DNA提取试剂盒 |
| 20021 | QIAEX II Gel Extraction Kit (150) | 胶回收纯化DNA |
| 12143 | QIAGEN Plasmid Midi Kit (25) | 质粒中提试剂盒 |
| 27104 | QIAprep Spin Miniprep Kit (50) | 提取高纯度质粒DNA |
| 28704 | QIAquick Gel Extraction Kit (50) | 胶回收试剂盒 |
| 28104 | QIAquick PCR Purification Kit (50) | PCR回收试剂盒 |
| 37900 | Qproteome Bacterial Protein Prep Kit | 细菌蛋白制备试剂盒 |
| 76106 | RNAlater (250ml) | RNAlater保护液 |
| 76104 | RNAlater (50ml) | RNAlater保护液 |
| 79254 | RNase-Free DNase Set (50) | DNA降解酶 |
| 74106 | RNeasy Mini Kit (250) | 细胞组织RNA提取试剂盒 |
| 74104 | RNeasy Mini Kit (50) | 细胞组织RNA提取试剂盒 |
| 74904 | RNeasy Plant Mini Kit (50) | 细胞组织RNA提取试剂盒 |
睿安公司专业技术服务
荧光定量PCR、甲基化检测、文库构建、原核和真核蛋白表达、高通量测序、抗体定制服务、WB检测、IHC检测。
睿安公司代理及优势品牌：
免疫学：Abgent、CST、R&D、Abcam、BD、Epitomics、Santa cruz、Jackson
耗材类： Ammex、Fisher、Bioplastics、Axygen、Corning、Greiner，Nunc-Nalgene
分子和细胞：Fermentas、hyclone-pierce、Qiagen、Bio-Rad
综合类： Sigma、 MERCK、Millipore、Roche、Invitrogen
自主品牌：Rainbio 生化类：Sigma-Vetec 、 MP
肥胖代谢类：Biovendor、ALPCO
表观遗传：Zymo ACTIVE MOTIF 神经和信号传导：Tocris
其他类： Biovision、Biocolor、Covance、Cytoskeleton、ebioscience、GE、Genetex、Invivogen、Omega、
Proteintech 、Socorex 、Vector
码RNA？mRNA还有不翻译的RNA呢。应该说转录水平指的是RNA水平，而蛋白应该叫翻译水平？我们这里就特指编码蛋白的mRNA的量的检测吧！方法：很多很多，我们常用的也就是RT-PCR和northern（实际上我也只知道他们，丢人啊），但是RNA没保护分析在国外很常用，也有半定量的功能，一次性试剂盒还能以此将一个表达簇一起分析，例如NFkB的转录激活谱中的8个常见基因。至于dot blot和原位杂交之流则实属于吃力不讨好，不知所云，骗人玩玩的啦，dot blot的最大贡献是发展到了反向dot blot
二聚体，非特异性扩增，可以通过调整引物的量、模板的量、镁离子来解决。若没有目的条带，调整引物的量、模板的量、镁离子纯属瞎闹。 3. 模板 提取总RNA和逆转录时大家只要严格按照试剂盒的说明书操作就行了，没什么技巧。提取总RNA我用英俊（invitrogen）的Trizol，量一定要够。逆转录我用fermentas（MBI）的逆转录试剂盒（RevertAid First Strand cDNA Synthesis Kit），货号K1622，很好用，加好样后，按42度60分钟、70度
RT2 Profiler PCR Arrays新手小技巧及注意事项
给QIAGEN技术人员，我们会帮您分析您的RNA样品是否适合进行PCR array。cDNA合成：我推荐以1 ug的总RNA为模板，用RT2 First Strand Kit (Cat # 330401)试剂盒进行cDNA合成。根据我们的经验这是最佳的起始反应用量。假如RNA量不够，起始总RNA样品量最少可以减至400 ng，不过必须保证RNA的质量足够好；假如连400 ng的RNA样品都无法保证，请联系QIAGEN技术人员，您可能需要进行预扩增。请使用相同起始量的RNA进行cDNA合成及后继实验，以保证
