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文献和实验Temperature-Induced Phase Partitioning for Protein Purification
Ethylene oxide (EO)-propylene oxide (PO) random copolymers are watersoluble and separate into two phases above a lower critical solution temperature (LCST), i.e., the cloud point, a polymer-rich phase, and a water phase depleted of polymer
!_ it follows that there is no such thing as a "2% (or any other %) paraformaldehyde" solution. Paraformaldehyde is a high polymer, and its molecules are too big to dissolve in water, alcohol or anything else. You have to depolymerize paraformaldehyde
RNA Intramolecular Dynamics by Single‐Molecule FRET
., ) 30 U/µL T4 DNA ligase HC (Fermentas) DNase I (Fermentas) Water‐saturated phenol (Carl Roth GmbH
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