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文献和实验Purification Scheme for Ubiquitin Expression Lysate
Purification Scheme for Ubiquitin Expression Lysate Step 1: Take the expression lysis supernatent and treat with concentrated acetic acid until the pH drops to between 4.5 and 5.0. The solution will turn milky white
into a clean SS-34 tube through a small two-ply square of cheesecloth placed in the center of a funnel. The cheese cloth catches any fragments of SDS/genomic DNA pellet floating on the surface.7. Precipitate the nucleic acids by adding 20 ml of isopropanol
while precipitating the genomic DNA and SDS in an insoluble white, rubbery precipitate. Shake bottle thoroughly. Centrifuge at 10 k × g for 10 minutes. 6. Pour the supernatant into a clean SS-34 tube through a small two-ply square of cheesecloth placed
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