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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
SUPERTIP-96, GRADUATED, 300UL
- 供应商:
西宝生物
- 保存条件:
ROOM TEMPERATURE
- 库存:
1 each
- 规格:
LABWARE
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文献和实验96 Well Mating Protocol platewise
on the XGAL plate 2. Streak recipient plasmid (pMSCV) on LB Spec50 CB100 0.2% glucose OR YEGlu spec50 cb100) Grow at 30oC overnight Protocol 1. Inoculate 5 ml single colony culture of recipient, and a 1:100 donor culture in 500 ul/well 96 well culture box
96 Well Mating Protocol platewise
, 100ul recipient pool is aliquotted to is added to each well of Costar U-bottom 96 well plate. Then each well gets either 1-2ul of donor overnight culture. Donor dilution: Acceptable donor OD600 range is 0.060 to 0.300 . Measure the cultures straight
96-well Plate Dual Luciferase Assay--96孔板荧光素酶检测
and resuspend the cells using the 1ml pipette. Trans fer about 50 ul to 200 ul out of 300ul to the 96 well plate depending on the confluency of each well. ( just to make sure the cell numbers of different wells are within 4 fold range) Spin down the cells
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