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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
多克隆
- 抗体英文名:
H3K9me2 polyclonal antibody (Histone H3)
- 规格:
25 ug
产品名称:H3K9me2多克隆抗体|H3K9me2 polyclonal antibody (Histone H3)
【产品形式】:艾美捷为您提供的H3K9me2多克隆抗体产品为无色溶液形式,含有25 ug抗体.该产品浓度为与批次相关,溶解于包含有0.05%NaN3和0.05% ProClin 300的PBS缓冲液中.请在收到该H3K9me2多克隆抗体产品后,将其置于-20 °C冷冻保存,建议分装使用以避免反复冻融.
【背景资料】:Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Dimethylation of histone H3K9 is more present in silent genes.
BioVision的公司总部在美丽的旧金山湾地区,它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有 卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品,以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代 谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理, 艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.
点击:H3K9me2多克隆抗体,查看更详细产品说明,更多应用、储存、价格、货期等信息请致电400-6800-868垂询BioVision中国区域授权总代理艾美捷科技有限公司。
关键词:H3K9me2,H3K9me2多克隆抗体
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文献和实验Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
Chromatin immunoprecipitation: optimization, quantitative analysis and data normalization
with antibodies against H3K9me2 and H3K27me2 (Figure 5 and data not shown). These data show that this sequence can be used as a positive ChIP control for histone modifications associated with active chromatin, and a negative control for histone modifications
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