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- 保质期:
1年
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大量
- 供应商:
无锡莱弗思生物实验器材有限公司
- 规格:
50 µl
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文献和实验PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
to chromatin, by its histone tail interaction or by other interactions, then you would not expect to see loss of binding upon peptide competition. A good positive control for the assay is to use H3K9 dimethylated peptide to compete HP1 from the pericentric
Chromatin Immunoprecipitation Protocol for Histone Modification
. This has been very successful when using antibodies that detect site specific histone lysine methylation (Peters et al . 2003, Martens et al . 2005). However, for each different antibody the optimal concentration should be tested and calibrated. The stringency of washing
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