- 询价
- Amresco
- 中国/美国/德国
- XY292802
- 2025年07月15日
13 年钻石会员
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- 详细信息
- 技术资料
- 保质期：
6个月
- 英文名：
SuperScript II Reverse Transcriptase
- 库存：
大量
- 供应商：
上海信裕
Super Script Ⅱ阴性逆转录酶
SuperScript II Reverse Transcriptase
BR
产地 中国/美国/德国
所在目录 酶类
产品性质及属性
级别 BR
来源 Purified from E. coli expressing the pol gene of M-MLV (1,2), mutagenized to reduce the RNase H activity
浓度 200u/ul
Super Script Ⅱ阴性逆转录酶活力定义 One unit of SuperScript® II is the amount of enzyme required to incorporate 1 nmole of eoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as template•primer (3).
Unit Reaction Conditions 50 mM Tris-HC1 (pH 8.3), 40 mM KC1, 6 mM MgC1 2 , 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18 , 0.1 mg/m1 BSA, and enzyme in 50 µl for 10 min. at 37℃
Performance and Quality Testing SDS-PAGE purity；endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
Enzyme Function RNA dependent DNA Polymerase
Final Product Size 12.3 kb or less
Number of Reactions 10 Reactions
Optimal Reaction Temperature 42℃
Sensitivity Medium
Template ssRNA；ssDNA；RNA-DNA Hybrid
产品描述 SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations rather than a deletion in the RNase H active center. Like SuperScript® RT, SuperScript® II RT has reduced RNase H activity. Unlike SuperScript® RT, however, the selective mutations within the RNase H domain maintain full polymerase activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics, including
性状 悬浮液
Super Script Ⅱ阴性逆转录酶用途 生化研究。
保存 -20℃
XYF115810 Fmoc-L-谷氨酰胺, 98%
XYF115873 N-甲酰-L-蛋氨酸 , 97%
XYF115876 Fmoc-L-烯丙基甘氨酸, 98%
XYF115881 芴甲氧羰酰基D-Β环己基丙氨酸, 98%
XYF115882 2-氟-L-苯丙氨酸, 99%
XYF115885 BOC-L-2-氟苯丙氨酸, 98%
XYF115940 FMOC-L-Β-同型亮氨酸, 96%
XYF116765 N-Fmoc-N'-三苯甲基-L-组氨酸五氟苯基酯, 75%
XYF116766 Fmoc-D-丙氨酸 , 98%
XYF116768 Fmoc-Arg(Mtr)-OH, 98%
XYF116771 Nα-Fmoc-Nω-甲苯磺酰基-L-精氨酸, 98%
XYF116772 Fmoc-D-天冬酰胺, 98%
XYF116773 Fmoc-L-天冬氨酸 alpha-烯丙酯, 97%
XYF116774 Fmoc-L-天冬氨酸 beta-叔丁酯, 98%
XYF116776 芴甲氧羰基-S-乙酰氨甲基-L-半胱氨酸, 98%
XYF116778 Fmoc-S-叔丁基-L-半胱氨酸, 98%
XYF116779 Fmoc-L-谷氨酸γ苄脂, ≥98.0% (HPLC)
XYF116780 N-芴甲氧羰基-D-谷氨酸 gamma-叔丁酯, 98%
XYF116782 N-Fmoc-N'-三苯甲基-L-组氨酸, 98%
XYF116787 Fmoc-D-异亮氨酸, 98.5%
XYF116788 Fmoc-D-亮氨酸, 98%
XYF116791 Fmoc-N'-三氟乙酰基-L-赖氨酸, 96%
XYF116793 Fmoc-D-脯氨酸, 98%
XYF116796 Fmoc-O-叔丁基-D-丝氨酸, 98%
XYF116797 Fmoc-O-苄基-L-丝氨酸, 98%
XYF116798 FMOC-O-叔丁基-L-丝氨酸 , 98%
XYF116799 Fmoc-O-三苯甲基-L-丝氨酸, 98%
XYF116801 Fmoc-L-色氨酸(Boc)-OH, 97.0%
XYF116802 Fmoc-L-酪氨酸, 97%
SuperScript II Reverse Transcriptase
BR
产地 中国/美国/德国
所在目录 酶类
产品性质及属性
级别 BR
来源 Purified from E. coli expressing the pol gene of M-MLV (1,2), mutagenized to reduce the RNase H activity
浓度 200u/ul
Super Script Ⅱ阴性逆转录酶活力定义 One unit of SuperScript® II is the amount of enzyme required to incorporate 1 nmole of eoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as template•primer (3).
Unit Reaction Conditions 50 mM Tris-HC1 (pH 8.3), 40 mM KC1, 6 mM MgC1 2 , 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18 , 0.1 mg/m1 BSA, and enzyme in 50 µl for 10 min. at 37℃
Performance and Quality Testing SDS-PAGE purity；endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
Enzyme Function RNA dependent DNA Polymerase
Final Product Size 12.3 kb or less
Number of Reactions 10 Reactions
Optimal Reaction Temperature 42℃
Sensitivity Medium
Template ssRNA；ssDNA；RNA-DNA Hybrid
产品描述 SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations rather than a deletion in the RNase H active center. Like SuperScript® RT, SuperScript® II RT has reduced RNase H activity. Unlike SuperScript® RT, however, the selective mutations within the RNase H domain maintain full polymerase activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics, including
性状 悬浮液
Super Script Ⅱ阴性逆转录酶用途 生化研究。
保存 -20℃
XYF115810 Fmoc-L-谷氨酰胺, 98%
XYF115873 N-甲酰-L-蛋氨酸 , 97%
XYF115876 Fmoc-L-烯丙基甘氨酸, 98%
XYF115881 芴甲氧羰酰基D-Β环己基丙氨酸, 98%
XYF115882 2-氟-L-苯丙氨酸, 99%
XYF115885 BOC-L-2-氟苯丙氨酸, 98%
XYF115940 FMOC-L-Β-同型亮氨酸, 96%
XYF116765 N-Fmoc-N'-三苯甲基-L-组氨酸五氟苯基酯, 75%
XYF116766 Fmoc-D-丙氨酸 , 98%
XYF116768 Fmoc-Arg(Mtr)-OH, 98%
XYF116771 Nα-Fmoc-Nω-甲苯磺酰基-L-精氨酸, 98%
XYF116772 Fmoc-D-天冬酰胺, 98%
XYF116773 Fmoc-L-天冬氨酸 alpha-烯丙酯, 97%
XYF116774 Fmoc-L-天冬氨酸 beta-叔丁酯, 98%
XYF116776 芴甲氧羰基-S-乙酰氨甲基-L-半胱氨酸, 98%
XYF116778 Fmoc-S-叔丁基-L-半胱氨酸, 98%
XYF116779 Fmoc-L-谷氨酸γ苄脂, ≥98.0% (HPLC)
XYF116780 N-芴甲氧羰基-D-谷氨酸 gamma-叔丁酯, 98%
XYF116782 N-Fmoc-N'-三苯甲基-L-组氨酸, 98%
XYF116787 Fmoc-D-异亮氨酸, 98.5%
XYF116788 Fmoc-D-亮氨酸, 98%
XYF116791 Fmoc-N'-三氟乙酰基-L-赖氨酸, 96%
XYF116793 Fmoc-D-脯氨酸, 98%
XYF116796 Fmoc-O-叔丁基-D-丝氨酸, 98%
XYF116797 Fmoc-O-苄基-L-丝氨酸, 98%
XYF116798 FMOC-O-叔丁基-L-丝氨酸 , 98%
XYF116799 Fmoc-O-三苯甲基-L-丝氨酸, 98%
XYF116801 Fmoc-L-色氨酸(Boc)-OH, 97.0%
XYF116802 Fmoc-L-酪氨酸, 97%
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Super Script Ⅱ阴性逆转录酶
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