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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Anti-Human CD34 FITC
- 规格：
25tests
to a protein family which also includes endoglycan and podocalyxin. Members of this family
are single pass transmembrane proteins with a heavily glycosylated extracellular and N-terminal
mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors,
and has since been extensively used as a marker to isolate cells capable of hematopoietic cell
engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression
on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells,
tumor cell lines, and by vascular endothelia in the adult. Epitopes of CD34 have been assigned to
three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage
by neuraminidase, chymopapain, or O-glycoprotease. According to this analysis, the 4H11 antibody
belongs to class III, indicating that it reacts with a protein epitope.
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文献和实验Transmigration of Human CD34+ Cells
to understand the mechanisms responsible for their homing and/or mobilization. In this chapter, we will describe the methodology utilized in our laboratory to evaluate migration of human CD34+ cells that contain HSC.
Generation of TransgenicT Cells from Human CD34+Cord Blood Cells
Hematopoietic stem cells (HSCs), progenitor cells capable of generating all peripheral blood cell lineages, are believed not to express lineage markers (Lin) found on the latter, nor CD38, but may or may not express CD34. Besides HSCs
Short-Term Culture of Human CD34+ Cells for Lentiviral Gene Transfer
biological properties of HSCs. We describe here methods for short-term ex vivo culture and gene transfer into human HSCs. Cord blood-derived HSC gene transfer can be tuned to limit the average level of vector integration or instead to maximize
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