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- BD
- 553126
- 2025年09月20日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
FITC Rat Anti-Mouse Ly-6G and LY-6C
- 规格：
0.1mg
BD Pharmingen
Alternative Name
Ly6c, Lymphocyte antigen 6C2; Lymphocyte antigen 6G, Ly6g, Gr-1
Concentration
0.5 mg/ml
Isotype
Rat IgG2b, κ
Reactivity
Mouse (QC Testing)
Application
Flow cytometry (Routinely Tested)
Immunogen
Not Reported
Description
The RB6-8C5 monoclonal antibody recognizes a common epitope on Ly-6G and Ly-6C, previously known as the myeloid differentiation antigen Gr-1. In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. The antigen is also expressed on the monocyte lineage in the bone marrow, but not on erythroid cells. In the periphery, RB6-8C5 antibody recognizes granulocytes (neutrophils and eosinophils) and monocytes. The RB6-8C5 antibody is a component of the "lineage cocktail" used in studies of hematopoietic cell lineages. The 1A8 antibody (Cat. No. 551461) specifically recognizes Ly-6G, but not Ly-6C.
Based on comparison of the staining patterns given by 1A8 versus RB6-8C5 antibodies on total blood leucocytes, it is evident that the 1A8 antibody stains the RB6-8C5-bright population, corresponding to Ly-6G-expressing granulocytes; whereas, the RB6-8C5-dim population is 1A8-negative and corresponds to Ly-6C-expressing lymphocytes and monocytes. Please refer to the Technical Data Sheets for Cat. No. 551459 and 553128 for more detailed information.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Format
- Format
- FITC
- Excitation Source
- Blue 488 nm
- Excitation Max
- 494 nm
- Emission Max
- 520 nm
FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.
Suggested Companion Products
| Cat No. | Description | Size | |
|---|---|---|---|
| 553988 | FITC Rat IgG2b, κ Isotype Control RUO | 0.25 mg |
|
| 553141 | Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) 2.4G2 RUO | 0.1 mg |
|
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- 作者
- 内容
- 询问日期
文献和实验olivial 最近在做细胞内信号转导通路PI-3K-Akt，关于它的抑制剂LY 294002的配制，说明书上写用DMSO，在做实验时设了vehicle（含等量DMSO）组，但发现DMSO对细胞（小胶质细胞）有影响，且DMSO与干预药有协同作用，MTT结果显示DMSO浓度>=0.1%即对细胞造成损伤，而任何浓度DMSO（0.01%~0.1%）都与干预药有协同作用，乙醇的结果亦如此。所以想请教，大家在配LY时是怎么配的？在使用LY时是否遇到了上述问题
Generation of Short-Term Murine Natural Killer Cell Clones to Analyze Ly49 Gene Expression
Natural killer (NK) cellsexpress receptors specific for class I major histocompatibility complex (MHC) molecules. In the mouse, the class I specific receptors identified to date belong to the polymorphic Ly49 receptor family. Engagement of Ly49
Hybrid Resistance by Mouse NK Cells In Vitro
of monoclonal antibodies (MAbs) or F(ab′)2 reagents to block those signals. There is already good evidence for negative signals between Ly49 NK cell receptors and their specific class I antigens, e.g., Ly49A and H2-Dd (2 ). Thus, the inability of H2b Ly49
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