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文献和实验To successfully perform molecular genetic techniques it is essential to have a full understanding of the properties of the various Escherichia coli host strains commonly used for the propagation and manipulation of recombinant DNA. E. coli
Use of tRNA-Supplemented Host Strains for Expression of Heterologous Genes in E. coli
in the heterologous gene from the codon usage of the E. coli host. The general problem arises form the fact that due to the redundancy of the genetic code one amino acid can be encoded by more than one codon and different organisms do not utilize each codon
Experimental Evolution of an Essential Bacillus Gene in an E. coli Host
into a single E. coli genotype. Wild-type E. coli is generally incapable of utilizing sucrose, but E. coli transformants were able to grow on sucrose as a sole carbon and energy source, albeit poorly. Twelve replicate populations were initiated and propagated
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