Use of tRNA-Supplemented Host Strains for Expression of Heterologous Genes in E. coli
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| Table 1 Codon Usage of Rare E. coli Codons in Other Organismsa | |||||
| Codon | AGG/AGA | CGA | CUA | AUA | CCC | 
| (cognate tRNA gene) | (argU ) | (leuW ) | (ileX/ileY ) | (proL ) | |
| Encoded amino acid | Arginine | Arginine | Leucine | Isoleucine | Proline | 
| E. coli K12 | 1.2/2.1 | 3.6 | 3.9 | 4.4 | 5.5 | 
| Homo sapiens | 11.5/11.3 | 6.3 | 7.0 | 7.2 | 20.0 | 
| Drosphila melanogaster | 6.4/5.1 | 8.5 | 8.3 | 9.3 | 17.9 | 
| S. cerivisiae | 9.3/21.3 | 3.0 | 13.4 | 17.8 | 6.8 | 
| Plasmodium falciparium | 4.0/16.7 | 2.4 | 5.3 | 44.3 | 2.6 | 
| Pyrococcus furiosus | 20.5/29.5 | 0.6 | 16.2 | 34.8 | 8.8 | 
| Thermus aquaticus | 14.5/1.4 | 1.6 | 3.6 | 1.4 | 39.3 | 
| Arabidopsis thaliana | 10.9/19.0 | 6.3 | 10.1 | 13.1 | 5.2 | 
| Triticum aestivum | 11.8/6.5 | 3.2 | 6.3 | 6.1 | 14.4 | 
| a Codon frequencies are presented as codons encountered in 1000 codons of coding sequence. This table was compiled from the codon usage web site (www.kazusa.or.jp/codon). If every codon would be evenly presented, a codon frequency of 15 would be expected. | 










