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- 文献和实验
- 技术资料
- 英文名:
BioLux Cypridina Luciferase Starter Kit
- 供应商:
南京赛泓瑞
- 规格:
100 assays
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文献和实验pGL3 Luciferase Reporter Vectors
The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA
Preparation of Nuclear Extracts for Gel Shifts and Westerns Blots
Centrifuge at 4 C at top speed (15000 x g ) for 3 min. 6. Place tubes on ice. 7. Save supernatant (cytosolic fraction) for luciferase/ß-gal assay, if desired. Otherwise, discard the supernatant
Affinity chromatography (with HIS-tagged proteins)
=flowthrough; Luc=luciferase, negative control).
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