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赖氨酰肽链内切酶 Lysyl Endopeptidase(R)

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赖氨酰肽链内切酶 Lysyl Endopeptidase(R)

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  • 货号: 129-02541,125-02543
    保存条件: -20℃冷冻
    供应商: Wako(和光纯药工业株式会社)
    英文名: Lysyl Endopeptidase®
    规格: 1vial (10AU) /1vial (2AU)
    规格: 1vial (10AU) 产品价格: 询价
    规格: 1vial (2AU) 产品价格: 询价

    Lysyl Endopeptidase, originally isolated from the soil bacterium discovered by Masaki, et al. cleaves specifically the peptide bonds at the carboxy-terminal side of Lysine residues and S-aminoethylcysteine residues with a high degree of specificity, making it a valuable tool for protein sequence analysis and for enzymatic synthesis of Lys-X compounds. An added feature of Lysyl Endopeptidase is its ability to retain complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 degrees C.
     

    • Appearance: Lyophilized form containing ca. 10% Tris-HCl buffer, pH 8.
    • Activity: Shown on each label
    • Molecular Weight: 27,000 (gel filtration); 30,000 (SDS electrophoresis)
    • Solubility: Soluble in water or buffer solution.
    • Stability: Stable at 4 degrees C, when dissolved in buffer of pH 5~12. Stable at 30 degrees C in the range of pH 6~11, but unstable at 50 degrees C or higher.
    • Optimal pH: 9.0 -9.5 (Amidase activity)
    • Isoelectric point: 6.9~7.0
    • Substrate specificity:
      Hydrolysable substrate ... Tos-Lys-Ome, Bz-Lys-NH2, Bz-Lys-pNA, Lys-pNA
      Unhydrolysable substrate ... Bz-Arg-NH2, Bz-Arg-pNa, Arg-pNA
    • Inhibitors: DFP, PMSF, TLCK

    Unit definition: One amidase unit (AU) is the amount of enzyme, which will produce 1 micromole of p-Nitroaniline per minute at 30 degrees C, pH 9.5.
    Source: Bacteria


    Originally, the source of this product was indicated as "Achromobacter lyticus" based on the physiological and morphological properties of the bacteria. However, we confirmed that the 16SrDNA sequence was highly homologous to that of Lysobacter

    温馨提示:不可用于临床治疗。

    资料下载:

    129-02541.pdf 附件下载 (下载 21 次)

  • 赖氨酰肽链内切酶 Lysyl Endopeptidase(R)

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