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赖氨酰肽链内切酶 Lysyl Endopeptidase(R)

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Wako(和光纯药工业株式会社)
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成立时间:1922
入驻丁香通年限:4年
商家等级:金牌客户
产品信息完整度:44%
联系人:代理商宝柏

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产品详情

品牌: Wako
货号: 129-02541,125-02543
保存条件: -20℃冷冻
供应商: Wako(和光纯药工业株式会社)
规格: 1vial (10AU) /1vial (2AU)
规格: 1vial (10AU) 产品价格: 询价
规格: 1vial (2AU) 产品价格: 询价

Lysyl Endopeptidase, originally isolated from the soil bacterium discovered by Masaki, et al. cleaves specifically the peptide bonds at the carboxy-terminal side of Lysine residues and S-aminoethylcysteine residues with a high degree of specificity, making it a valuable tool for protein sequence analysis and for enzymatic synthesis of Lys-X compounds. An added feature of Lysyl Endopeptidase is its ability to retain complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 degrees C.
 

  • Appearance: Lyophilized form containing ca. 10% Tris-HCl buffer, pH 8.
  • Activity: Shown on each label
  • Molecular Weight: 27,000 (gel filtration); 30,000 (SDS electrophoresis)
  • Solubility: Soluble in water or buffer solution.
  • Stability: Stable at 4 degrees C, when dissolved in buffer of pH 5~12. Stable at 30 degrees C in the range of pH 6~11, but unstable at 50 degrees C or higher.
  • Optimal pH: 9.0 -9.5 (Amidase activity)
  • Isoelectric point: 6.9~7.0
  • Substrate specificity:
    Hydrolysable substrate ... Tos-Lys-Ome, Bz-Lys-NH2, Bz-Lys-pNA, Lys-pNA
    Unhydrolysable substrate ... Bz-Arg-NH2, Bz-Arg-pNa, Arg-pNA
  • Inhibitors: DFP, PMSF, TLCK

Unit definition: One amidase unit (AU) is the amount of enzyme, which will produce 1 micromole of p-Nitroaniline per minute at 30 degrees C, pH 9.5.
Source: Bacteria


Originally, the source of this product was indicated as "Achromobacter lyticus" based on the physiological and morphological properties of the bacteria. However, we confirmed that the 16SrDNA sequence was highly homologous to that of Lysobacter

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资料下载: 129-02541.pdf 附件下载 (下载20 次)

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品牌:santacruze  货号:sc-224481  价格:询价 
Lysyl Endopeptidase, originally isolated from the soil bacterium discovered by Masaki, et al. cleaves specifically the peptide bonds at the carboxy-terminal side of Lysine residues and S-aminoethylcysteine residues with a high degree of specificity, making it a valuable tool for protein sequence analysis and for enzymatic synthesis of Lys-X compounds. An added feature of Lysyl Endopeptidase is its ability to retain complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 degrees C.  Appearance: Lyophilized form containing ca. 10% Tris-HCl buffer, pH 8. Activity: Shown on each label Molecular Weight: 27,000 (gel filtration); 30,000 (SDS electrophoresis) Solubility: Soluble in water or buffer solution. Stability: Stable at 4 degrees C, when dissolved in buffer of pH 5~12. Stable at 30 degrees C in the range of pH 6~11, but unstable at 50 degrees C or higher. Optimal pH: 9.0 -9.5 (Amidase activity) Isoelectric point: 6.9~7.0 Substrate specifwako129-02541,125-02543
Wako  125-02543 Lysyl Endopeptidase? 现货 【产品名称】,Wako  125-02543 Lysyl Endopeptidase? 现货【制造商】Wako Pure Chemical Industries, Ltd.【销售商代码】125-02543【规格】1vial(2AU)【价格】咨询 4OO-811-9081【分子量】27,500【保存条件】Keep at RT.【外观】Wako  125-02543 Lysyl Endopeptidase? 现货【注意事项】This product is for research use only. Do not administer it to human.Lysyl Endopeptidase, originally isolated from the soil bacterium discovered by Masaki, et al. cleaves specifically the peptide bonds at the carboxy-terminal side of Lysine residues and S-aminoethylcysteine residues with a high degree of specificity, making it a valuable tool for protein sequence analysis and for enzymatic synthesis of Lys-X compounds. An added feature of Lysyl Endopeptidase is its ability to retain complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 degrees C.Unit definition: One amidase unit (AU) is the amount of enzyme, which will produce 1 micromole of p-Nitroaniline per minute at 3ziqibio125-02543
Lysyl Endopeptidase;Achromobacter protase)From Achromobacter lyticus M497-1. Supplied as a lyophilized, white powder, free of salts. Each vial contains 10 units, in 1ml Tris-HCl, 2mM, pH 8.0.Activity: <126>4 units/mgCatalyzes cleavage of the peptide bonds at the carboxy-terminal of lysine residues.Unit Definition: The amount of enzyme required for the formation of 1 mmol of p-nitroaniline per minute at 30<144>C pH 9.5.Ref.: 1. Masaki, T., Nakamura, K., Isono, M. and Soejima, M., Agricultural and Biological Chemistry, 42: 1443-5 (1978). 2. Masaki, T., Fujihashi, T., Nakamura, K. and Soejima, M., Biochem. Biophys. Acta., 660: 44 (1981). 3. Masaki, T., Fujihashi, T., Nakamura, K. and Soejima, M., Biochem. Biophys. Acta., 660: 51 (1981). 4. Morihara, K., Oka, T., Tsuzaki, H., Tochino, Y. and Kanaya, T., Biochemical and Biophysical Research Communications, 92: 396-402 (1980). 5. Masaki, T. and Soejima, M., Agricultural and Biological Chemistry, 49: 1867-8 (1985). 6. Makano, K., Tashimp0215389810
Lysyl Endopeptidase(R) for Biochemistry125-02543-110 1 vial (2 AU)129-02541-110 1 vial (10 AU)上海甄准生物科技有限公司www.zzstandard.com电话:021-6840 4353传真:021-5897 9353客服:13795317828 QQ:2453006496  手机QQ:13795317828客服:15800340161 QQ:648862165客服:13512172575 QQ:1745420292SummaryThis product is for research use only. Do not administer it to human.Purity: Homogenous on SDS-PAGEActivity: shown on each label. Unit: AU*/vial* AU: Amidase Unit - amount of enzyme required for the formation of 1 umol of p-nitroaniline fromN-benzoyl-DL-lysine-p-nitroaniline per minute at 30 degrees C, pH 9.5.Preparation A 10AU-vial contains Tris-HCl corresponding to 1 mL of 2 mM Tris HCl buffer (pH 8.0).Molecular Weight] 27,000 (gel filtration); 30,000 (SDS electrophoresis)Optimal pH 9.0 -9.5 (amidase activity)Stability Very stable in lyophilized forms at -20 degrees C; Stable for at least 2 years at -20 degrees Cwhen dissolved in Tris-HCl buffer at pH 4.0 to 11 at the enzyme concentration of more than zzstandard125-02543-110

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