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- 2025年07月14日
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文献和实验When recombinant glycoproteins for therapeutic use are to be produced on an industrial scale, there is a crucial need for technologies that can engineer fast-growing stable cells secreting the protein drug at a high rate and with a defined
up 300 ml of CHO medium to 37 deg.C and add cytochalasin B (150 ul of 10 mg/ml) and nocodazole (300 ul of 10 mg/ml) Make sure all buffers are in coldroom, there is rocker in the coldroom and there is a good aspirator in the coldroom. Hook up a sawed
Establishment of Stable Transfectant of CHO Lec Cells
Purpose and Backgrounds CHO lec 3.2.8.1 cells CHO Lec 3.2.8.1 cells have four independent mutations in the N- and O- glycosylation pathways (Stanley, 1989). N-linked carbohydrates produced by CHO Lec 3.2.8.1 cells are all of the high
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