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- 详细信息
- 文献和实验
- 技术资料
- 库存：
500 g
- 保存条件：
ROOM TEMPERATURE
- 英文名：
2xYT BACTERIOLOGICAL MEDIUM
- 供应商：
西宝生物
- 规格：
CELL BIOLOGY
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文献和实验Recipe for yeast tryptone medium
Recipe for yeast tryptone medium Keywords: Zappe; YT 2x; Yeast tryptone; E. coli Yeast tryptone medium Contributor: Zappe, H Related: E
Transforming chemically competent cells
: According to the original TSS paper and qualitative experience (JM), this step is completely optional and may actually reduce transformation efficiency. Incubate cells on ice for 2 min. Add 1 mL SOC (2XYT and LB are also suitable, original paper suggests
to seal and allow to cool before use Eleven liters 2xYT in 4- 4 L flasks, 45 min exposure Eight 500 ml bottles Tubing for harvesting 10% antifoam 289, final concentration in media: 0.01% Start o/n of AMA
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