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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
西宝生物
- 英文名：
PLATE COUNT BROTH, m
- 库存：
500 g
- 保存条件：
ROOM TEMPERATURE
- 规格：
CELL BIOLOGY
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文献和实验Preparation of Broth and Plates, etc.
Recipes: 1) LB Broth Make 16 gm of LB Broth Base (Gibco #M27800C) up to 800 ml in ddH2O. Swirl to dissolve, then add 110 µl of 10 N NaOH. Autoclave. 2) NZY Broth
Preparation of Broth and Plates
Preparation of Broth and Plates, etc. [Use ddH2O 17 m* or better ] Recipes: 1) LB Broth Make 16 gm of LB Broth Base (Gibco #M27800C) up to 800 ml in ddH2O. Swirl to dissolve, then add 110 µl of 10 N NaOH
siRNA transfection protocol (6 well plate)
75 μ l OMEM + 2.5 μ l SilentFect (BioRad). Mix gently by pipetting 3x. Sit RT 2-5 mins. 75 μ l OMEM + 1.1 μ l siRNA (20 μ M stock => 20 nM final). Mix gently by pippetting 3x. Combine siRNA and transfection solution
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