Anti-HumanHost: sheepForm: purified antiserumConc/Titer: 1:3,000-1:15,000Applications: ELISA; Immunoblotting; Indirect ImmunofluorescenceThis polyclonal antibody developed in rabbit is highly specific against HIV-I Rev protein. Rev is an essential regulatory protein of HIV-I. The immunogen was prepared as glutathione-s-transferase fusion protel expressed in vitro (E. coli). This antibody recognizes wild type Rev protein produced both in vivo (expression in cos.cv-1 and HeLa cell lines) and in vitro (bacterial expression system and in vitro translation products). Extremely low cross reactivity with other E. coli proteins is observed on western blot. Anti-HIV Rev (PAB) recognizes epitopes from both human and rat sources. The tyrosine kinase activity of immunoprecipitated antigen is retained and higher immunoprecipitation is observed than with monoclonal anti-Rev.