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- 详细信息
- 技术资料
- 保存条件:
-20 C
- 供应商:
西宝生物
- 英文名:
HIV REVPOLYCLONAL ANTIBODY
- 库存:
100 µg
- 规格:
IMMUNOBIOLOGICALS
Anti-Human
Host:
sheep
Form:
purified antiserum
Conc/Titer:
1:3,000-1:15,000
Applications:
ELISA; Immunoblotting; Indirect Immunofluorescence
This polyclonal antibody developed in rabbit is highly specific against HIV-I Rev protein. Rev is an essential regulatory protein of HIV-I. The immunogen was prepared as glutathione-s-transferase fusion protel expressed
in vitro (E. coli
). This antibody recognizes wild type Rev protein produced both
in vivo
(expression in cos.cv-1 and HeLa cell lines) and
in vitro
(bacterial expression system and
in vitro
translation products). Extremely low cross reactivity with other
E. coli
proteins is observed on western blot. Anti-HIV Rev (PAB) recognizes epitopes from both human and rat sources. The tyrosine kinase activity of immunoprecipitated antigen is retained and higher immunoprecipitation is observed than with monoclonal anti-Rev.
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HIV REVPOLYCLONAL ANTIBODY
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