Anti-Vimentin is delipidized antiserum raised in goat by repeated injections of purified vimentin from cultured human foreskin fibroblasts. The antibody shows broad species cross reactivity and no reactivity with other intermediate filame nts. The product is an antiserum to vimentin, the intermediate filament subunit of mesenchymal cells and stains specifically the vimentin band at a molecular weight of 58,000 using total cell or tissue extracts in the immunoblotting technique.