DESCRIPTION Glutathione is a tripeptide of glycine, glutamic acid and cysteine. In the red blood cell, the reduced form of glutathione is vital in maintaining hemoglobin in a reduced state and hence protecting the cells from oxidative damage. Glutathione is involved in detoxification of hydrogen peroxide through glutathione oxidase. Low levels of glutathione are found in deficiencies of key enzymes involved in glutathione metabolism, such as glucose-6-phosphate dehydrogenase, glutathione synthase and glutathione reductase. Simple, direct and automation-ready procedures for measuring reduced glutathione are becoming popular in Research and Drug Discovery. Our Assay Kit is designed to accurately measure reduced glutathione in biological samples. The optical density of the formed yellow product, measured at 412 nm, is directly proportional to glutathione concentration in the sample.
KEY FEATURES Sensitive and accurate. Linear detection range 0.4 - 100 μM in 96-well plate. Simple and convenient. The procedure involves mixing the Reagent with sample, removing protein precipitates for proteinaceous samples, adding a second Reagent and reading the optical density. Low interference. Amino acids and common buffers do not interfere.
APPLICATIONS Direct Assays: reduced glutathione in whole blood, plasma, serum, urine, tissue and cell extracts. Drug Discovery/Pharmacology: effects of drugs on glutathione metabolism.
STORAGE CONDITIONS The kit is shipped at room temperature. Store all components at 4°C. Shelf life: 6 months.