DESCRIPTION β-GLUCOSIDASE is a glucosidase enzyme which acts upon β-1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). β-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a β-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a β-glucosidase isoform (lysosomal glucocerebrosidase) has been related to Gauchers disease and Parkinsons disease. Simple, direct and automation-ready procedures for measuring β-glucosidase activity are becoming popular in Research and Drug Discovery. Our β-Glucosidase Assay Kit is designed to measure β-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes a substrate that is hydrolyzed specifically by β-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
KEY FEATURES High sensitivity and wide linear range. Use 20 μL sample. The detection limit is 2 U/L, linear up to 250 U/L. Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of α-glucosidase activity within 20 minutes.
Robust and amenable to HTS. All reagents are compatible with highthroughput liquid handling instruments.
APPLICATIONS 1. Direct Assays: α-glucosidase activity in biological samples. 2. Characterization and Quality Control for α-glucosidase production. 3. Drug Discovery: high-throughput screen and evaluation of α-glucosidase inhibitors.
STORAGE CONDITIONS The kit is shipped at room temperature. Store all components at -20°C. Shelf life of at least 6 months.