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- Invitrogen
- AM2082
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
T7 RNA Polymerase (cloned) 20 U/μL
- 库存：
1
- 供应商：
研卉生物
- 规格：
5000units
T7 RNA Polymerase (cloned) 20 U/μL
(Invitrogen™)
Unit Definition:
One unit of RNA Polymerase will catalyze the incorporation of 1 nmol of nucleoside triphosphate into acid-insoluble material in 60 min at 37°C.
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文献和实验mRNA Amplification with T7 RNA Polymerase
on a 42ºC oven and set PCR machine to hold at 70ºC. In an Eppendorf tube add: 1 µg Total RNA 1 µL T7 oligo(dT) primer q.s. to 12 µL with Nuclease-free H2 O Incubate 10 min. at 70ºC. Spin briefly to pull
Oocyte Expression With Injection of Purified T7 RNA Polymerase
of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.
T7 RNA Polymerase-Mediated Incorporation of 8-N3AMP Into RNA for Studying Protein-RNA Interactions
Ultraviolet (UV)-dependent photochemical crosslinking is a powerful approach that can be used for the identification of RNA-protein interactions. Although 8-azidoATP (8-N3 ATP) has been widely used to elucidate the ATP binding site
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